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Literature summary for 3.4.19.9 extracted from
- Gamma-glutamyl hydrolase conjugase. Purification and properties of the bovine hepatic enzyme (1975), J. Biol. Chem., 250, 5982-5994.
General Stability
| General Stability | Organism |
|---|---|
| stability against various dialysis procedures | Bos taurus |
| zinc is essential for stability | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | - |
Bos taurus |  |
| concanavalin A | - |
Bos taurus | |
| Cu2+ | - |
Bos taurus | |
| Dextran sulfate | - |
Bos taurus | |
| DNA | - |
Bos taurus | |
| Fe3+ | - |
Bos taurus | |
| heparin | - |
Bos taurus | |
| Hg2+ | - |
Bos taurus | |
| Mn2+ | - |
Bos taurus | |
| NEM | - |
Bos taurus | |
| PCMB | - |
Bos taurus | |
| RNA | - |
Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0017 | - |
Pteroyltriglutamate | - |
Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| lysosome | - |
Bos taurus | 5764 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zinc | contains 4.15-4.47 zinc atoms per molecule of enzyme | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 108000 | - |
gel filtration | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Bos taurus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 5.0 mM 2-mercaptoethanol, 40% loss of activity after 24 h | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polyglutamylfolate + H2O | bond cleavage occurs with equal facility at internal points of the peptide chain | Bos taurus | ? | - |
? | |
| polyglutamylfolate + H2O | longer chain gamma-glutamyl peptides are preferentially attacked by the enzyme | Bos taurus | ? | - |
? | |
| polyglutamylfolate + H2O | peptide bond cleavage occurs only at gamma-glutamyl bonds and the presence of a COOH-terminal gamma bond is essential for enzyme action | Bos taurus | ? | - |
? | |
| polyglutamylfolate + H2O | the cleavage of diglutamyl peptides is extremely slow | Bos taurus | ? | - |
? | |
| polyglutamylfolate + H2O | the cleavage of gamma bonds is independent of the NH2-terminal pteroyl moiety | Bos taurus | ? | - |
? | |
| pteroyltriglutamate + H2O | - |
Bos taurus | ? | - |
? | |
| pteroyltriglutamate + H2O | - |
Bos taurus | pteroylglutamate + glutamate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Bos taurus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
little thermal denaturation below | Bos taurus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | pteroyltriglutamate: 732 gamma-glutamyl bonds are cleaved per min per mol of enzyme | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.9 | - |
sodium acetate buffer | Bos taurus |
| 4.5 | - |
sodium citrate buffer | Bos taurus |
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Release 2023.1 (January 2023)
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