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Literature summary for 3.4.19.5 extracted from

  • Cantor, J.R.; Stone, E.M.; Chantranupong, L.; Georgiou, G.
    The human asparaginase-like protein 1 hASRGL1 is an Ntn hydrolase with beta-aspartyl peptidase activity (2009), Biochemistry, 48, 11026-11031.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.19.5

Cloned(Commentary)

Cloned (Comment) Organism
expressed as a His-tagged fusion protein in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
T168A mutant does not show enzymatic activity Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14550
-
 mass spectroscopy, beta-subunit Homo sapiens
18000
-
 Western blot, reducing conditions, detected in soluble and insoluble fractions Homo sapiens
18500
-
 mass spectroscopy, alpha-subunit Homo sapiens
33000
-
 Western blot, reducing conditions, detected in soluble and insoluble fractions Homo sapiens
33020
-
 mass spectroscopy, precursor enzyme Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q7L266
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-NTA chromatography. hASRGL1 purification by immobilized metal ion affinity chromatography yields 30 mg of protein/l with a purity of more than 90% as determined by SDS-PAGE Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-L-Asp-L-Ala + H2O
-
 Homo sapiens L-Asp + L-Ala
-
 ?
beta-L-Asp-L-Leu + H2O
-
 Homo sapiens L-Asp + L-Leu
-
 ?
beta-L-Asp-L-Lys + H2O
-
 Homo sapiens L-Asp + L-Lys
-
 ?
beta-L-Asp-L-Phe + H2O
-
 Homo sapiens L-Asp + L-Phe
-
 ?
beta-L-Asp-L-Phe methyl ester + H2O
-
 Homo sapiens L-Asp + L-Phe methyl ester
-
 ?
L-Asp beta-methyl ester + H2O
-
 Homo sapiens L-Asp + methanol
-
 ?
L-aspartic acid beta-(7-amido-4-methylcoumarin) + H2O
-
 Homo sapiens L-aspartic acid + 7-amino-4-methylcoumarin
-
 ?

Synonyms

Synonyms Comment Organism
asparaginase-like protein 1
-
 Homo sapiens
hASRGL1
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens