Activating Compound | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | weak activation | Gallus gallus | |
DFP | weak activation | Gallus gallus | |
EDTA | weak activation | Gallus gallus | |
PMSF | weak activation | Gallus gallus |
General Stability | Organism |
---|---|
2-mercaptoethanol and EDTA stabilize | Gallus gallus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | - |
Gallus gallus | |
Hg2+ | - |
Gallus gallus | |
iodoacetate | - |
Gallus gallus | |
L-trans-epoxysuccinyl-leucylamide-(4-guanido)-butane | i.e. E-64, partial | Gallus gallus | |
Ni2+ | - |
Gallus gallus | |
PCMB | - |
Gallus gallus | |
peptidic calpain inhibitor | i.e. EP-475, partial | Gallus gallus | |
pyroglutamyl-D-Ala | - |
Gallus gallus | |
pyroglutamyl-Pro | - |
Gallus gallus | |
Sodium tetrathionate | - |
Gallus gallus | |
Zn2+ | - |
Gallus gallus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
pyroglutamate-4-methylcoumarin 7-amide | - |
Gallus gallus | |
0.7 | - |
pyroglutamyl-2-naphthylamide | - |
Gallus gallus | |
0.73 | - |
pyroglutamyl-p-nitroanilide | - |
Gallus gallus | |
0.89 | - |
L-pyroglutamyl-L-Ala | - |
Gallus gallus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
86000 | - |
gel filtration | Gallus gallus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gallus gallus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Gallus gallus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Gallus gallus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.45 | - |
- |
Gallus gallus |
Storage Stability | Organism |
---|---|
-20°C, rapid inactivation in presence of EDTA and 2-mercaptoethanol | Gallus gallus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bradykinin potentiator + H2O | - |
Gallus gallus | pyroglutamate + ? | - |
? | |
L-pyroglutamyl-L-Ala + H2O | - |
Gallus gallus | L-pyroglutamate + L-Ala | - |
? | |
litorin + H2O | frog litorin | Gallus gallus | pyroglutamate + Gln-Trp-Ala-Val-Gly-His-Phe-Met-NH2 | - |
? | |
luteinizing-hormone-releasing hormone + H2O | - |
Gallus gallus | ? | - |
? | |
additional information | the enzyme is inactive towards uperolein and piperidone carbonyl His-Pro-NH2 | Gallus gallus | ? | - |
? | |
N-(4-methyl-2-oxo-2H-chromen-7-yl)-5-oxo-L-prolinamide + H2O | - |
Gallus gallus | 5-oxo-L-proline + 7-amino-4-methylcoumarin | - |
? | |
neurotensin + H2O | - |
Gallus gallus | pyroglutamate + Leu-Tyr-Glu-Asn-Lys-Pro-Ag-Arg-Pro-Tyr-Ile-Leu | - |
? | |
physalaemin + H2O | - |
Gallus gallus | pyroglutamate + Ala-Asp-Pro-Asn-Lys-Phe-Tyr-Gly-Leu-Met-NH2 | - |
? | |
pyroglutamyl-beta-naphthylamide + H2O | - |
Gallus gallus | pyroglutamate + beta-naphthylamine | - |
? | |
pyroglutamyl-D-Ala + H2O | slight activity | Gallus gallus | pyroglutamate + D-Ala | - |
? | |
pyroglutamyl-p-nitroanilide + H2O | - |
Gallus gallus | pyroglutamate + p-nitroaniline | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
pH 7.8, 15 min, stable | Gallus gallus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | hydrolysis of pyroglutamyl-2-naphthylamide or pyroglutamyl-Ala | Gallus gallus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | 30°C, 30 min, stable in presence of 5 mM each of EDTA and 2-mercaptoethanol | Gallus gallus |
7 | 8.2 | stable | Gallus gallus |