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Literature summary for 3.4.19.3 extracted from
- Pyrrolidone carboxyl peptidase from the hyperthermophilic archaeon Pyrococcus furiosus: cloning and overexpression in Escherichia coli of the gene, and its application to protein sequence analysis (1998), J. Biochem., 124, 778-783.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| overexpression in Escherichia coli | Pyrococcus furiosus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C142S | complete loss of the activity | Pyrococcus furiosus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | - |
Pyrococcus furiosus |  |
| Hg2+ | - |
Pyrococcus furiosus | |
| N-ethylmaleimide | 1 mM, activity is completely lost | Pyrococcus furiosus | |
| p-chloromercuribenzoate | 1 mM, activity is completely lost | Pyrococcus furiosus | |
| Zn2+ | - |
Pyrococcus furiosus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 22936 | - |
x * 22936, ionspray triple quadrupole mass spectrometry, carboxymethylated enzyme | Pyrococcus furiosus |
| 22940 | - |
ionspray triple quadrupole mass spectrometry, carboxymethylated enzyme | Pyrococcus furiosus |
| 45640 | - |
ionspray triple quadrupole mass spectrometry, native enzyme | Pyrococcus furiosus |
| 45643 | - |
x * 45643, ionspray triple quadrupole mass spectrometry, native enzyme | Pyrococcus furiosus |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| guanidine-HCl | approximately 70% of the original activity is retained after preincubation with 10 mM DTT, 50 mM Na-phosphate buffer (pH 7.0) containing less than either 0.01% SDS, 1 M urea, or 1 M guanidine-HCl at 37°C for 15 min | Pyrococcus furiosus |
| SDS | approximately 70% of the original activity is retained after preincubation with 10 mM DTT, 50 mM Na-phosphate buffer (pH 7.0) containing less than either 0.01% SDS, 1 M urea, or 1 M guanidine-HCl at 37°C for 15 min | Pyrococcus furiosus |
| urea | approximately 70% of the original activity is retained after preincubation with 10 mM DTT, 50 mM Na-phosphate buffer (pH 7.0) containing less than either 0.01% SDS, 1 M urea, or 1 M guanidine-HCl at 37°C for 15 min | Pyrococcus furiosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | O73944 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pyrococcus furiosus |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 22936, ionspray triple quadrupole mass spectrometry, carboxymethylated enzyme | Pyrococcus furiosus |
| ? | x * 45643, ionspray triple quadrupole mass spectrometry, native enzyme | Pyrococcus furiosus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PCP | - |
Pyrococcus furiosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 90 | - |
- |
Pyrococcus furiosus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | 105 | 75°C: about 60% of maximal activity, 105°C: about 90% of maximal activity | Pyrococcus furiosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
150 min, more than 80% of the activity is retained | Pyrococcus furiosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 9 | - |
Pyrococcus furiosus |
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