Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | rapid increase in activity from 2 to 6 mM, above this concentration plateau effect | Bos taurus |
Cloned (Comment) | Organism |
---|---|
PAP1 gene cloned into vector pCR2.1, subcloned into vector pQE60, subcloned into expression vector pPC225, having the ORF for recombinant PAP1 in fusion with a His 6 tag on the C-terminal, expressed in Escherichia coli XL10-Gold cells | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
pyroglutamic acid-Ala | competitive inhibitor | Bos taurus | |
pyroglutamic acid-His-Pro-NH2 | thyrotropin-releasing hormone, relative highest affinity, competitive inhibitor | Bos taurus | |
pyroglutamic acid-Val | competitive inhibitor | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.059 | - |
pyroglutamic acid-7-amido-4-methylcoumarin | - |
Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
1 * 24000, SDS-PAGE | Bos taurus |
24020 | - |
sequence analysis | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by immobilised nickel ion affinity chromatography | Bos taurus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
340 | - |
crude enzyme | Bos taurus |
3633 | - |
purified enzyme | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyroglutamic acid-7-amido-4-methylcoumarin + H2O | - |
Bos taurus | L-pyroglutamate + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 24000, SDS-PAGE | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
PAP1 | - |
Bos taurus |
pyroglutamyl peptidase type-1 | - |
Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.5 | - |
pyroglutamic acid-7-amido-4-methylcoumarin | - |
Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
9.0-9.5 | Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 10 | activity dropping off sharply outside this pH range | Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0441 | - |
pyroglutamic acid-His-Pro-NH2 | - |
Bos taurus | |
0.141 | - |
pyroglutamic acid-Ala | - |
Bos taurus | |
0.6522 | - |
pyroglutamic acid-Val | - |
Bos taurus |