Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.19.3 extracted from

  • Kilbane, Z.; Vaas, P.R.; Cuiv, P.O.; OConnor, B.
    Cloning and heterologous expression of bovine pyroglutamyl peptidase type-1 in Escherichia coli: purification, biochemical and kinetic characterisation (2007), Mol. Cell. Biochem., 297, 189-197.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol rapid increase in activity from 2 to 6 mM, above this concentration plateau effect Bos taurus

Cloned(Commentary)

Cloned (Comment) Organism
PAP1 gene cloned into vector pCR2.1, subcloned into vector pQE60, subcloned into expression vector pPC225, having the ORF for recombinant PAP1 in fusion with a His 6 tag on the C-terminal, expressed in Escherichia coli XL10-Gold cells Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
pyroglutamic acid-Ala competitive inhibitor Bos taurus
pyroglutamic acid-His-Pro-NH2 thyrotropin-releasing hormone, relative highest affinity, competitive inhibitor Bos taurus
pyroglutamic acid-Val competitive inhibitor Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.059
-
pyroglutamic acid-7-amido-4-methylcoumarin
-
Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
1 * 24000, SDS-PAGE Bos taurus
24020
-
sequence analysis Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
by immobilised nickel ion affinity chromatography Bos taurus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
340
-
crude enzyme Bos taurus
3633
-
purified enzyme Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyroglutamic acid-7-amido-4-methylcoumarin + H2O
-
Bos taurus L-pyroglutamate + 7-amino-4-methylcoumarin
-
?

Subunits

Subunits Comment Organism
monomer 1 * 24000, SDS-PAGE Bos taurus

Synonyms

Synonyms Comment Organism
PAP1
-
Bos taurus
pyroglutamyl peptidase type-1
-
Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Bos taurus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5
-
pyroglutamic acid-7-amido-4-methylcoumarin
-
Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
9.0-9.5 Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
7.5 10 activity dropping off sharply outside this pH range Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0441
-
pyroglutamic acid-His-Pro-NH2
-
Bos taurus
0.141
-
pyroglutamic acid-Ala
-
Bos taurus
0.6522
-
pyroglutamic acid-Val
-
Bos taurus