Activating Compound | Comment | Organism | Structure |
---|---|---|---|
thio-reducing agent | absolute requirement | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed by use of a baculovirus vector in Spodoptera frugiperda | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Pyrrolidone | reversible | Homo sapiens | |
Ca2+ | 1 mM, 20% inhibition | Homo sapiens | |
Cu2+ | 1 mM, 96% inhibition | Homo sapiens | |
E64 | 0.1 mM, 15% inhibition | Homo sapiens | |
NEM | reversible | Homo sapiens | |
Ni2+ | 0.1 mM, 98% inhibition | Homo sapiens | |
Zn2+ | 1 mM, 98% inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
pyroglutamyl-4-methylcoumaryl-7-amide | pH 7.8, 37°C | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23188 | - |
1 * 23188, calculation from nucleotide sequence | Homo sapiens |
24000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9NXJ5 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
colon | mucosa | Homo sapiens | - |
mucosa | from colon | Homo sapiens | - |
Storage Stability | Organism |
---|---|
-20°C, retains more than 90% of its activity for 1 month in the presence of a thiol-reducing agent | Homo sapiens |
4°C, pH 7.8, stable for at least 1 week | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ACE inhibitor + H2O | contains the N-terminal dipeptide pyroglutamyl-Trp- | Homo sapiens | pyroglutamate + ? | - |
? | |
bombesin + H2O | contains the N-terminal dipeptide pyroglutamyl-Arg- | Homo sapiens | pyroglutamate + ? | - |
? | |
bradykinin potentiator B + H2O | contains the N-terminal dipeptide pyroglutamyl-Gly- | Homo sapiens | pyroglutamate + ? | - |
? | |
leukopyrokinin + H2O | contains the N-terminal dipeptide pyroglutamyl-Thr- | Homo sapiens | pyroglutamate + ? | - |
? | |
luteinizing hormone releasing hormone + H2O | contains the N-terminal dipeptide pyroglutamyl-His- | Homo sapiens | pyroglutamate + ? | - |
? | |
neurotensin + H2O | contains the N-terminal dipeptide pyroglutamyl-Leu- | Homo sapiens | pyroglutamate + ? | - |
? | |
pyroglutamyl-4-methylcoumaryl-7-amide + H2O | - |
Homo sapiens | pyroglutamate + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 23188, calculation from nucleotide sequence | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
PGPEP1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
4.5 h, stable up to | Homo sapiens |
50 | - |
90 min, enzyme is completely stable in crude preparation | Homo sapiens |
60 | - |
30 min, activity is almost completely lost | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | hydrolysis of pyroglutamyl-4-methylcoumaryl-7-amide | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | pH 6.0: about 75% of maximal activity, pH 9.0: about 70% of maximal activity, hydrolysis of pyroglutamyl-4-methylcoumaryl-7-amide | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
NEM | pH 7.8, 37°C | Homo sapiens | |
0.05 | - |
2-Pyrrolidone | pH 7.8, 37°C | Homo sapiens |