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Literature summary for 3.4.19.2 extracted from
- Carboxamidopeptidase: purification and characterization of a neurohypophyseal hormone inactivating peptidase from toad skin (1980), Biochemistry, 19, 39-48.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-methylumbelliferyl p-guanidinobenzoate | - |
toad |  |
| antipain | - |
toad | |
| chymostatin | - |
toad | |
| concanavalin A | - |
toad | |
| diisopropyl phosphofluoridate | - |
toad | |
| Elastatinal | - |
toad | |
| leupeptin | - |
toad | |
| Lima bean trypsin inhibitor | - |
toad | |
| ovoinhibitor | - |
toad | |
| ovomucoid | - |
toad | |
| p-Nitrophenyl p-guanidinobenzoate | - |
toad | |
| PCMB | reversed by Cys | toad | |
| tosyllysine chloromethyl ketone | - |
toad | |
| tosylphenylalanine chloromethyl ketone | - |
toad | |
| Trasylol | - |
toad | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
2 * 48000, each subunit consists of a heavy chain, 28000 da, and a light chain, 19000 Da joined by a disulfide bond, SDS-PAGE | toad |
| 100000 | - |
gel filtration | toad |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | toad | enzyme inactivates neurophyseal hormones | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| toad | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
toad |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| skin | - |
toad | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-L-Trp ethyl ester + H2O | - |
toad | ? | - |
? | |
| benzoyl-L-Arg ethyl ester + H2O | - |
toad | ? | - |
? | |
| additional information | enzyme inactivates neurophyseal hormones | toad | ? | - |
? | |
| oxytocin + H2O | hydrolysis of the Leu8-Gly9-NH2 bond | toad | glycinamide + ? | - |
? | |
| [Arg8]-vasopressin + H2O | hydrolysis of the Arg8-Gly9-NH2 bond | toad | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 48000, each subunit consists of a heavy chain, 28000 da, and a light chain, 19000 Da joined by a disulfide bond, SDS-PAGE | toad |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
oxytocin | toad |
| 7.5 | 8.5 | hydrolysis of 8-arginine-vasopressin or benzoyl-L-Arg ethyl ester | toad |
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