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Literature summary for 3.4.19.14 extracted from

  • Carter, B.; Wiseman, A.; Orkiszewski, R.; Ballard, K.; Ou, C.; Lieberman, M.
    Metabolism of leukotriene C4 in gamma-glutamyl transpeptidase-deficient mice (1997), J. Biol. Chem., 272, 12305-12310.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
acivicin
-
Mus musculus
additional information GSH is not a competitive inhibitor of gamma-glutamyl leukotrienase, since even an 80fold molar excess of GSH in the reaction mixture does not alter the rate of cleavage of leukotriene C4 Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0022
-
leukotriene C4 in 0.1 M Tris-HCl buffer, pH 8.0, at 37°C Mus musculus
0.03
-
S-decyl-GSH in 0.1 M Tris-HCl buffer, pH 8.0, at 37°C Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Mus musculus 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
papain-cleaved enzyme, gel filtration Mus musculus
65000
-
papain-cleaved enzyme, sedimentation velocity centrifugation Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
Mus musculus
-
liver
-
Mus musculus
-
lung
-
Mus musculus
-
pancreas
-
Mus musculus
-
small intestine
-
Mus musculus
-
spleen highest activity in spleen Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
leukotriene C4 + H2O inclusion of Gly-Gly in the incubation mixture does not significantly enhance the conversion of leukotriene C4 to leukotriene D4 Mus musculus leukotriene D4 + L-glutamate
-
?
additional information the enzyme does not cleave GSH or GSSG, gamma-glutamyl-4-nitroanilide, and gamma-glutamyl-4-methoxy-2-naphthylamide Mus musculus ?
-
?
S-decyl-GSH + H2O the substrate has the strongest binding among all of the S-alkyl-GSH analyzed and has binding activity similar to leukotriene C4 Mus musculus ?
-
?

Synonyms

Synonyms Comment Organism
gamma-glutamyl leukotrienase
-
Mus musculus
GGL
-
Mus musculus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37 60 at 60°C for 5 min completely inactivates cleavage of LTC4, while at 37°C the enzyme is active for as long as 5 h Mus musculus