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Literature summary for 3.4.19.12 extracted from

  • Nikko, E.; Andre, B.
    Evidence for a direct role of the Doa4 deubiquitinating enzyme in protein sorting into the MVB pathway (2007), Traffic, 8, 566-581.
    View publication on PubMed

Application

Application Comment Organism
additional information direct role of Doa4 in multivesicular body sorting pathway that is linked to its catalytic activity. Doa4 mediates a deubiquitination step required for sorting of CPS into the multivesicular body pathway Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
C571S is unable to maintain normal ubiquitin levels. In doa4delta cells expressing C571S, CPS is missorted to the limiting membrane of the vacuole Saccharomyces cerevisiae
additional information multivesicular body sorting of Gap1 is defective in a doa4delta mutant, which is not suppressed by restoring the ubiquitin pool. In the doa4delta ypt6delta strain, the pool of monomeric ubiquitin is as reduced as in the single doa4delta mutant Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
endosome the important function of Doa4 in Gap1 endocytosis operates at the late endosome stage Saccharomyces cerevisiae 5768
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Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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-
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information deubiquitination by Doa4 of cargo proteins and/or some components of the multivesicular body sorting machinery is essential to correct sorting of cargoes into the multivesicular body pathway Saccharomyces cerevisiae ?
-
?
ubiquitin + H2O
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Saccharomyces cerevisiae ?
-
?

Synonyms

Synonyms Comment Organism
Doa4
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Saccharomyces cerevisiae
yeast ubiquitin hydrolase
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Saccharomyces cerevisiae