Application | Comment | Organism |
---|---|---|
additional information | direct role of Doa4 in multivesicular body sorting pathway that is linked to its catalytic activity. Doa4 mediates a deubiquitination step required for sorting of CPS into the multivesicular body pathway | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
C571S | is unable to maintain normal ubiquitin levels. In doa4delta cells expressing C571S, CPS is missorted to the limiting membrane of the vacuole | Saccharomyces cerevisiae |
additional information | multivesicular body sorting of Gap1 is defective in a doa4delta mutant, which is not suppressed by restoring the ubiquitin pool. In the doa4delta ypt6delta strain, the pool of monomeric ubiquitin is as reduced as in the single doa4delta mutant | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endosome | the important function of Doa4 in Gap1 endocytosis operates at the late endosome stage | Saccharomyces cerevisiae | 5768 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | deubiquitination by Doa4 of cargo proteins and/or some components of the multivesicular body sorting machinery is essential to correct sorting of cargoes into the multivesicular body pathway | Saccharomyces cerevisiae | ? | - |
? | |
ubiquitin + H2O | - |
Saccharomyces cerevisiae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Doa4 | - |
Saccharomyces cerevisiae |
yeast ubiquitin hydrolase | - |
Saccharomyces cerevisiae |