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Literature summary for 3.4.19.12 extracted from

  • Luchansky, S.J.; Lansbury, P.T.; Stein, R.L.
    Substrate recognition and catalysis by UCH-L1 (2006), Biochemistry, 45, 14717-14725.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00004
-
ubiquitin-7-amido-4-methylcoumarin with UCH-L1 synthetic construct
0.00008
-
ubiquitin-AW with UCH-L1 synthetic construct
0.0001
-
ubiquitin-W-R42A with UCH-L1 synthetic construct
0.0001
-
ubiquitin-W-G75A with UCH-L1 synthetic construct
0.0001
-
ubiquitin-W-R42A with UCH-L3 synthetic construct
0.00013
-
ubiquitin-W with UCH-L1 synthetic construct
0.0002
-
ubiquitin-W-R74A with UCH-L1 synthetic construct
0.0003
-
ubiquitin-W-K48A with UCH-L1 synthetic construct
0.00031
-
ubiquitin-W-I44A with UCH-L1 synthetic construct
0.0004
-
ubiquitin-W-K63A with UCH-L1 synthetic construct
0.0004
-
ubiquitin-W-I44A with UCH-L3 synthetic construct
0.0004
-
ubiquitin-W-G75A with UCH-L3 synthetic construct
0.00045
-
ubiquitin-WA with UCH-L1 synthetic construct
0.0005
-
ubiquitin-W-H68A with UCH-L1 synthetic construct
0.0005
-
ubiquitin-W-H68A with UCH-L3 synthetic construct
0.0006
-
ubiquitin-W-V70A with UCH-L1 synthetic construct
0.0006
-
ubiquitin-W-R72A with UCH-L1 synthetic construct
0.0007
-
ubiquitin-W-K48A with UCH-L3 synthetic construct
0.0008
-
ubiquitin-W-K63A with UCH-L3 synthetic construct
0.0009
-
ubiquitin-W-K6A with UCH-L1 synthetic construct
0.0011
-
ubiquitin-W-K11A with UCH-L1 synthetic construct
0.0011
-
ubiquitin-W-G76A with UCH-L3 synthetic construct
0.0011
-
ubiquitin-W-V70A with UCH-L3 synthetic construct
0.0014
-
ubiquitin-W-K6A with UCH-L3 synthetic construct
0.0019
-
ubiquitin-W-R72A with UCH-L3 synthetic construct
0.002
-
ubiquitin-W-G76A with UCH-L1 synthetic construct
0.0023
-
ubiquitin-W-K11A with UCH-L3 synthetic construct
0.0045
-
ubiquitin-W-R74A with UCH-L3 synthetic construct
0.0058
-
ubiquitin-W-L73A with UCH-L1 synthetic construct
0.008
-
ubiquitin-W-L71A with UCH-L1 synthetic construct
0.0104
-
ubiquitin-W-L73A with UCH-L3 synthetic construct
0.0198
-
ubiquitin-W-L71A with UCH-L3 synthetic construct
0.0282
-
ubiquitin-W-L8A with UCH-L3 synthetic construct
0.03
-
ubiquitin-W-L8A with UCH-L1 synthetic construct

Organism

Organism UniProt Comment Textmining
synthetic construct
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ubiquitin-7-amido-4-methylcoumarin + H2O
-
synthetic construct ubiquitin + 7-amino-4-methylcoumarin
-
?
ubiquitin-7-amido-4-methylcoumarin + H2O
-
synthetic construct ?
-
?
ubiquitin-AW + H2O
-
synthetic construct ?
-
?
ubiquitin-AW + H2O poor substrate for UCH-L1 synthetic construct ?
-
?
ubiquitin-W + H2O
-
synthetic construct ?
-
?
ubiquitin-W + H2O competent substrate for both UCH-L1 and UCH-L3 synthetic construct ?
-
?
ubiquitin-W-G75A + H2O
-
synthetic construct ?
-
?
ubiquitin-W-G76A + H2O
-
synthetic construct ?
-
?
ubiquitin-W-H68A + H2O
-
synthetic construct ?
-
?
ubiquitin-W-I44A + H2O
-
synthetic construct ?
-
?
ubiquitin-W-K11A + H2O
-
synthetic construct ?
-
?
ubiquitin-W-K48A + H2O
-
synthetic construct ?
-
?
ubiquitin-W-K63A + H2O
-
synthetic construct ?
-
?
ubiquitin-W-K6A + H2O
-
synthetic construct ?
-
?
ubiquitin-W-L71A + H2O
-
synthetic construct ?
-
?
ubiquitin-W-L73A + H2O
-
synthetic construct ?
-
?
ubiquitin-W-L8A + H2O
-
synthetic construct ?
-
?
ubiquitin-W-R42A + H2O
-
synthetic construct ?
-
?
ubiquitin-W-R72A + H2O
-
synthetic construct ?
-
?
ubiquitin-W-R74A + H2O
-
synthetic construct ?
-
?
ubiquitin-W-V70A + H2O
-
synthetic construct ?
-
?
ubiquitin-WA + H2O
-
synthetic construct ?
-
?
ubiquitin-WA + H2O poor substrate for UCH-L1 synthetic construct ?
-
?

Synonyms

Synonyms Comment Organism
ubiquitin carboxy-terminal hydrolase L1
-
synthetic construct
UCH-L1
-
synthetic construct
UCH-L3
-
synthetic construct

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0001
-
ubiquitin-AW with UCH-L1 synthetic construct
0.0005
-
ubiquitin-WA with UCH-L1 synthetic construct
0.0011
-
ubiquitin-W-G76A with UCH-L1 synthetic construct
0.01
-
ubiquitin-W-L73A with UCH-L1 synthetic construct
0.01
-
ubiquitin-W-R74A with UCH-L1 synthetic construct
0.01
-
ubiquitin-W-G75A with UCH-L1 synthetic construct
0.02
-
ubiquitin-W-R42A with UCH-L1 synthetic construct
0.02
-
ubiquitin-7-amido-4-methylcoumarin with UCH-L1 synthetic construct
0.03
-
ubiquitin-W-K6A with UCH-L1 synthetic construct
0.03
-
ubiquitin-W-K11A with UCH-L1 synthetic construct
0.03
-
ubiquitin-W-K63A with UCH-L1 synthetic construct
0.03
-
ubiquitin-W-L71A with UCH-L1 synthetic construct
0.03
-
ubiquitin-W-R72A with UCH-L1 synthetic construct
0.03
-
ubiquitin-W with UCH-L1 synthetic construct
0.04
-
ubiquitin-W-K48A with UCH-L1 synthetic construct
0.05
-
ubiquitin-W-V70A with UCH-L1 synthetic construct
0.06
-
ubiquitin-W-H68A with UCH-L1 synthetic construct
0.09
-
ubiquitin-W-I44A with UCH-L1 synthetic construct
0.2
-
ubiquitin-W-R42A with UCH-L3 synthetic construct
0.3
-
ubiquitin-W-R74A with UCH-L3 synthetic construct
1.6
-
ubiquitin-W-I44A with UCH-L3 synthetic construct
1.7
-
ubiquitin-W-G76A with UCH-L3 synthetic construct
2.4
-
ubiquitin-W-K48A with UCH-L3 synthetic construct
2.4
-
ubiquitin-W-K63A with UCH-L3 synthetic construct
2.9
-
ubiquitin-W-G75A with UCH-L3 synthetic construct
3.4
-
ubiquitin-W-K11A with UCH-L3 synthetic construct
4
-
ubiquitin-W-K6A with UCH-L3 synthetic construct
4.4
-
ubiquitin-W-R72A with UCH-L3 synthetic construct
4.6
-
ubiquitin-W-H68A with UCH-L3 synthetic construct
5.7
-
ubiquitin-W-L73A with UCH-L3 synthetic construct
6.6
-
ubiquitin-W-L8A with UCH-L3 synthetic construct
6.7
-
ubiquitin-W-V70A with UCH-L3 synthetic construct
9.2
-
ubiquitin-W-L71A with UCH-L3 synthetic construct