Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme activation modeling | Homo sapiens | |
additional information | enzymatic activation mediated by substrate binding | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
overexpression of wild-type and mutant GST-tagged enzymes in Escherichia coli | Homo sapiens |
subcloned from pcDNA-UCH-L1 vector into pGEX-6P-1 vector. The resulting N-terminally fused GST-tagged protein expressed in Escherichia coli Rosetta strain | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
at 2.4 A resolution, x-ray crystallography. Overall fold resembles that of other ubiquitin hydrolases, including UCHL3. Geometry of the catalytic residues in the active site of UCH-L1 is distorted in such a way that the hydrolytic activity appears to be impossible without substrate induced conformational rearrangements | Homo sapiens |
purified recombinant wild-type and mutant GST-tagged enzymes, 35 mg/ml protein in 50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 10 mM DTT, crystallization at room temperature, X-ray diffraction structure determination and analysis at 2.6 A resolution, molecular modeling, structure-function relationship | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
C220S | sie-directed mutagenesis, structure analysis in comparison to the wild-type enzyme | Homo sapiens |
S18Y | sie-directed mutagenesis, structure analysis in comparison to the wild-type enzyme | Homo sapiens |
S18Y | monomeric in solution | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P15374 | - |
- |
Purification (Comment) | Organism |
---|---|
by gel filtration | Homo sapiens |
recombinant wild-type and mutant GST-tagged enzymes from Escherichia coli by glutathione affinity chromatography and gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | specific for | Homo sapiens | - |
additional information | the enzyme is absent in tissues other than brain | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ubiquitin-7-amido-4-methylcoumarin + H2O | ubiquitin makes modest contact with UCH-L1. The interaction is mainly electrostatic, contributed by conserved acidic residues E7, E11, D30, E35, and E37 | Homo sapiens | ubiquitin + 7-amino-4-methylcoumarin | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | crystallography | Homo sapiens |
dimer | in crystal structure | Homo sapiens |
monomer | in solution, sedimentation equilibrium analysis | Homo sapiens |
monomer | in solution, sedimentation equilibrium experiments | Homo sapiens |
More | structure-function relationship | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
PGP9.5 | - |
Homo sapiens |
ubiquitin C-terminal hydrolase | - |
Homo sapiens |
ubiquitin hydrolase | - |
Homo sapiens |
UCH-L1 | - |
Homo sapiens |