Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ubiquitin | - |
Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km with several mutant ubiquitin substrates | Bos taurus | |
0.0033 | - |
wild-type di-ubiquitin | pH 8.0, 37°C | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | PA700 regulatory complex of 26S proteasome | Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
di-ubiquitin + H2O | active with wild-type and mutant di-ubiquitin, the latter contains a distal mutant ubiquitin L8A/I44A | Bos taurus | ubiquitin | - |
? | |
polyubiquitin + H2O | hydrolysis is restricted to the distal end of the substrate, i.e. ubiquitin farthest from the site of protein attachment in poly-ubiquitin conjugates | Bos taurus | ubiquitin | - |
? | |
polyubiquitin + H2O | active with Lys48-, Lys6-, and Lys11-linked poly-ubiquitins | Bos taurus | ubiquitin | - |
? | |
polyubiquitin + H2O | no activity with poly-ubiquitins that contain a distal mutant ubiquitin L8A/I44A | Bos taurus | ubiquitin | - |
? | |
wild-type di-ubiquitin + H2O | - |
Bos taurus | ubiquitin | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | rate with several ubiquitin mutant substrates | Bos taurus | |
0.0728 | - |
wild-type di-ubiquitin | pH 8.0, 37°C | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Bos taurus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
ubiquitin | pH 8.0, 37°C | Bos taurus |