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Literature summary for 3.4.19.12 extracted from

  • Matsui, S.; Sandberg, A.A.; Negoro, S.; Seon, B.K.; Goldstein, G.
    Isopeptidase: a novel eukaryotic enzyme that cleaves isopeptide bonds (1982), Proc. Natl. Acad. Sci. USA, 79, 1535-1539.
    View publication on PubMedView publication on EuropePMC

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Gallus gallus 5737
-
cytoplasm
-
Cricetulus griseus 5737
-
cytoplasm
-
Mammalia 5737
-
cytoplasm
-
eukaryota 5737
-
cytoplasm
-
Saccharomyces cerevisiae 5737
-
cytoplasm
-
amphibia 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
divalent cations most divalent cations, except Mg2+ and Ca2+ are inhibitory Gallus gallus
divalent cations most divalent cations, except Mg2+ and Ca2+ are inhibitory Cricetulus griseus
divalent cations most divalent cations, except Mg2+ and Ca2+ are inhibitory Mammalia
divalent cations most divalent cations, except Mg2+ and Ca2+ are inhibitory eukaryota
divalent cations most divalent cations, except Mg2+ and Ca2+ are inhibitory Saccharomyces cerevisiae
divalent cations most divalent cations, except Mg2+ and Ca2+ are inhibitory amphibia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
gel filtration Cricetulus griseus

Organism

Organism UniProt Comment Textmining
amphibia
-
-
-
Cricetulus griseus
-
-
-
eukaryota
-
-
-
Gallus gallus
-
-
-
Mammalia
-
-
-
no activity in Escherichia coli
-
-
-
Saccharomyces cerevisiae
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
DON cell chinese hamster cell line DON Cricetulus griseus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Cricetulus griseus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
conjugate of histone H2A and ubiquitin + H2O i.e. A24, structural chromatin protein, activity of the enzyme is confined to conjugates between ubiquitin and other proteins, formed through epsilon-(alpha-glycyl)lysine bonds Gallus gallus ubiquitin + histone H2A
-
?
conjugate of histone H2A and ubiquitin + H2O i.e. A24, structural chromatin protein, activity of the enzyme is confined to conjugates between ubiquitin and other proteins, formed through epsilon-(alpha-glycyl)lysine bonds Cricetulus griseus ubiquitin + histone H2A
-
?
conjugate of histone H2A and ubiquitin + H2O i.e. A24, structural chromatin protein, activity of the enzyme is confined to conjugates between ubiquitin and other proteins, formed through epsilon-(alpha-glycyl)lysine bonds Mammalia ubiquitin + histone H2A
-
?
conjugate of histone H2A and ubiquitin + H2O i.e. A24, structural chromatin protein, activity of the enzyme is confined to conjugates between ubiquitin and other proteins, formed through epsilon-(alpha-glycyl)lysine bonds eukaryota ubiquitin + histone H2A
-
?
conjugate of histone H2A and ubiquitin + H2O i.e. A24, structural chromatin protein, activity of the enzyme is confined to conjugates between ubiquitin and other proteins, formed through epsilon-(alpha-glycyl)lysine bonds Saccharomyces cerevisiae ubiquitin + histone H2A
-
?
conjugate of histone H2A and ubiquitin + H2O i.e. A24, structural chromatin protein, activity of the enzyme is confined to conjugates between ubiquitin and other proteins, formed through epsilon-(alpha-glycyl)lysine bonds amphibia ubiquitin + histone H2A
-
?
additional information core histones and H1 are not cleaved Gallus gallus ?
-
?
additional information core histones and H1 are not cleaved Cricetulus griseus ?
-
?
additional information core histones and H1 are not cleaved Mammalia ?
-
?
additional information core histones and H1 are not cleaved eukaryota ?
-
?
additional information core histones and H1 are not cleaved Saccharomyces cerevisiae ?
-
?
additional information core histones and H1 are not cleaved amphibia ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Gallus gallus
37
-
assay at Cricetulus griseus
37
-
assay at Mammalia
37
-
assay at eukaryota
37
-
assay at Saccharomyces cerevisiae
37
-
assay at amphibia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
Gallus gallus
8 8.5
-
Cricetulus griseus
8 8.5
-
Mammalia
8 8.5
-
eukaryota
8 8.5
-
Saccharomyces cerevisiae
8 8.5
-
amphibia