Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | - |
Bos taurus | |
iodoacetamide | - |
Homo sapiens | |
Ubiquitin aldehyde | - |
Bos taurus | |
ubiquitin-aldehyde | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
gel filtration | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Homo sapiens | - |
erythrocyte | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14.75 | - |
purified enzyme, substrate A24 | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
conjugate of histone H2A and ubiquitin + H2O | best substrate | Bos taurus | ubiquitin + histone H2A | - |
? | |
conjugate of histone H2A and ubiquitin + H2O | i.e. A24, structural chromatin protein, activity of the enzyme is confined to conjugates between ubiquitin and other proteins, formed through epsilon-(alpha-glycyl)lysine bonds | Bos taurus | ubiquitin + histone H2A | - |
? | |
additional information | enzyme contains ubiquitin-C-terminal hydrolase activity towards biosynthetic precursors of ubiquitin as well as isopeptidase activity towards ubiquitin histone conjugates as human ubiquitin carboxyl extension protein and ubiquitin C-terminal amide | Bos taurus | ? | - |
? | |
ubiquitin C-terminal amide + H2O | - |
Homo sapiens | ubiquitin + NH3 | - |
? | |
ubiquitin fused to 60% of its N-terminus + H2O | recombinant substrate, expressed in Escherichia coli | Bos taurus | ubiquitin + 60% of ubiquitin N-terminus | - |
? | |
ubiquitin metallothionein + H2O | low activity | Bos taurus | ? | - |
? | |
ubiquitin metallothionein + H2O | recombinant substrate, expressed in Escherichia coli | Bos taurus | ? | - |
? | |
ubiquitin metallothionein + H2O | i.e. UbMT | Bos taurus | ? | - |
? | |
ubiquitin-carboxyl extension protein + H2O | carboxyl extension protein of 52 amino acids | Homo sapiens | ubiquitin + ubiquitin-carboxyl extension protein | - |
? | |
ubiquitin-human carboxyl extension protein fusion protein + H2O | i.e. HUbCEP-52 | Bos taurus | ubiquitin + human carboxyl extension protein | - |
? | |
ubiquitin-human carboxyl extension protein fusion protein + H2O | recombinant substrate, expressed in Escherichia coli | Bos taurus | ubiquitin + human carboxyl extension protein | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
60 min, 70% loss of activity towards A24, no loss of activity towards ubiquitin C-terminal amide and human ubiquitin carboxyl extension protein | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Bos taurus |