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Literature summary for 3.4.19.11 extracted from

  • Garnier, M.; Vacheron, M.J.; Guinand, M.; Michel, G.
    Purification and partial characterization of the extracellular gamma-D-glutamyl-(L)meso-diaminopimelate endopeptidase I, from Bacillus sphaericus NCTC 9602 (1985), Eur. J. Biochem., 148, 539-543.
    View publication on PubMed
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Inhibitors

Inhibitors Comment Organism Structure
EDTA reactivation by zinc, cobalt and manganese ions Lysinibacillus sphaericus
o-phenanthroline reactivation by zinc, cobalt and manganese ions Lysinibacillus sphaericus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.57
-
 N-acetylmuramoyl-L-Ala-gamma-D-Glu-(L)-meso-diaminopimelic acid-(L)-D-Ala enzyme from sporulation medium Lysinibacillus sphaericus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Lysinibacillus sphaericus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activates apoenzyme, maximal activation at 0.0002 mM Lysinibacillus sphaericus
Mn2+ activates apoenzyme, maximal activation at 0.0001 mM Lysinibacillus sphaericus
Zn2+ zinc enzyme Lysinibacillus sphaericus
Zn2+ activates apoenzyme Lysinibacillus sphaericus
Zn2+ zinc-binding triad His69-Glu72-His196 Lysinibacillus sphaericus
Zn2+ maximal activation at 0.0002 mM Lysinibacillus sphaericus

Organism

Organism UniProt Comment Textmining
Lysinibacillus sphaericus
-
 NCTC 9602
-

Purification (Commentary)

Purification (Comment) Organism
-
 Lysinibacillus sphaericus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-Acetylmuramoyl-L-Ala-gamma-D-Glu-(L)-meso-diaminopimelic acid-(L)-D-Ala + H2O
-
 Lysinibacillus sphaericus N-Acetylmuramoyl-L-Ala-gamma-D-Glu + meso-diaminopimelic acid-(L)-D-Ala
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 stable for 45 days Lysinibacillus sphaericus
55
-
 10 min, 2% loss of activity Lysinibacillus sphaericus
65
-
 10 min, 3% loss of activity Lysinibacillus sphaericus
75
-
 10 min, 13% loss of activity Lysinibacillus sphaericus
80
-
-
 Lysinibacillus sphaericus
85
-
 10 min, 88% loss of activity Lysinibacillus sphaericus
95
-
 10 min, 99% loss of activity Lysinibacillus sphaericus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
 pI: 5.4 Lysinibacillus sphaericus