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Literature summary for 3.4.19.1 extracted from

  • Liu, C.; Yang, G.; Wu, L.; Tian, G.; Zhang, Z.; Feng, Y.
    Switch of substrate specificity of hyperthermophilic acylaminoacyl peptidase by combination of protein and solvent engineering (2011), Protein Cell, 2, 497-506.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.19.1

Protein Variants

Protein Variants Comment Organism
F488G/R526V/T560W 1.55fold increase in activity with 4-nitrophenyl laurate compared to activity of mutant R526V/T560W Aeropyrum pernix
additional information the esterase activity of the mutant R526V (this mutation transforms a promiscuous acylaminoacyl peptidase into a specific carboxylesterase) towards substrates with long acyl chains is enhanced by protein engineering and solvent optimization. The substrate preference of the enzyme can be further changed from 4-nitrophenyl octanoate to 4-nitrophenyl dodecanoate by protein and solvent engineering Aeropyrum pernix
R526V mutant enzyme with high esterase activity, extreme thermal stability, and high tolerance to organic solvents Aeropyrum pernix
R526V/T560W 1.5fold increase in activity with 4-nitrophenyl dodecanoate compared to activity of mutant R526V Aeropyrum pernix
W474V/F488G/R526V/T560W the mutant enzyme has 7fold higher catalytic efficiency (kcat/Km) for 4-nitrophenyl dodecanoate than the mutant enzyme R526V Aeropyrum pernix
W474V/R526V/T560W 3.11fold increase in activity with 4-nitrophenyl laurate compared to activity of mutant R526V/T560W Aeropyrum pernix

Organism

Organism UniProt Comment Textmining
Aeropyrum pernix Q9YBQ2
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Aeropyrum pernix DSM 11879 Q9YBQ2
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-Leu-4-nitroanilide + H2O switch of substrate specificity of hyperthermophilic promiscuous acylaminoacyl peptidase by combination of protein and solvent engineering into a specific carboxylesterase Aeropyrum pernix N-acetyl-L-Leu + 4-nitroaniline
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 ?
N-acetyl-Leu-4-nitroanilide + H2O switch of substrate specificity of hyperthermophilic promiscuous acylaminoacyl peptidase by combination of protein and solvent engineering into a specific carboxylesterase Aeropyrum pernix DSM 11879 N-acetyl-L-Leu + 4-nitroaniline
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 ?