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Literature summary for 3.4.19.1 extracted from

  • Palmieri, G.; Langella, E.; Gogliettino, M.; Saviano, M.; Pocsfalvi, G.; Rossi, M.
    A novel class of protease targets of phosphatidylethanolamine-binding proteins (PEBP): a study of the acylpeptide hydrolase and the PEBP inhibitor from the archaeon Sulfolobus solfataricus (2010), Mol. Biosyst., 6, 2498-2507.
    View publication on PubMed
Search for more literature for 3.4.19.1

Application

Application Comment Organism
medicine the enzyme may constitute a new therapeutic target for the treatment of a number of pathologies Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
CaCl2 100 mM, 52% inhibition Saccharolobus solfataricus
phosphatidylethanolamine-binding protein inhibitor SsCEI
-
 Saccharolobus solfataricus
SDS above 0.3 mM, complete inhibition Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
 N-acetyl-L-leucyl 4-nitroanilide pH 7.5, 80°C Saccharolobus solfataricus
11.4
-
 N-acetyl-L-alanyl 4-nitroanilide pH 7.5, 80°C Saccharolobus solfataricus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Saccharolobus solfataricus 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 2 * 60000, SDS-PAGE Saccharolobus solfataricus
125000
-
 gel filtration Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q7LX61
-
-
Saccharolobus solfataricus P2 Q7LX61
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.1
-
 pH 7.5, 80°C Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with L-leucyl 4-nitroanilide or L-alanyl 4-nitroanilide. The enzyme is able to hydrolyse N-succinyl-Gly-Gly-Phe 4-nitroanilide, showing endopeptidase activity Saccharolobus solfataricus ?
-
 ?
additional information no activity with L-leucyl 4-nitroanilide or L-alanyl 4-nitroanilide. The enzyme is able to hydrolyse N-succinyl-Gly-Gly-Phe 4-nitroanilide, showing endopeptidase activity Saccharolobus solfataricus P2 ?
-
 ?
N-acetyl-L-alanyl 4-nitroanilide + H2O
-
 Saccharolobus solfataricus N-acetyl-L-alanine + 4-nitroaniline
-
 ?
N-acetyl-L-alanyl 4-nitroanilide + H2O
-
 Saccharolobus solfataricus P2 N-acetyl-L-alanine + 4-nitroaniline
-
 ?
N-acetyl-L-leucyl 4-nitroanilide + H2O preference for N-acetyl-L-leucyl 4-nitroanilide over N-acetyl-L-alanyl 4-nitroanilide Saccharolobus solfataricus N-acetyl-L-leucine + 4-nitroaniline
-
 ?
N-acetyl-L-leucyl 4-nitroanilide + H2O preference for N-acetyl-L-leucyl 4-nitroanilide over N-acetyl-L-alanyl 4-nitroanilide Saccharolobus solfataricus P2 N-acetyl-L-leucine + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 60000, SDS-PAGE Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
acylpeptide hydrolase
-
 Saccharolobus solfataricus
APEH
-
 Saccharolobus solfataricus
apeH-2
-
 Saccharolobus solfataricus
sso2141
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 72 h, stable Saccharolobus solfataricus
70
-
 72 h, stable Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5
-
 N-acetyl-L-leucyl 4-nitroanilide pH 7.5, 80°C Saccharolobus solfataricus
5
-
 N-acetyl-L-alanyl 4-nitroanilide pH 7.5, 80°C Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 9 at 80°C Saccharolobus solfataricus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00002
-
 phosphatidylethanolamine-binding protein inhibitor SsCEI pH 7.5, 80°C, substrate: N-acetyl-L-leucyl 4-nitroanilide Saccharolobus solfataricus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.00000002
-
 pH 7.5, 80°C, substrate: N-acetyl-L-leucyl 4-nitroanilide Saccharolobus solfataricus phosphatidylethanolamine-binding protein inhibitor SsCEI

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.4
-
 N-acetyl-L-alanyl 4-nitroanilide pH 7.5, 80°C Saccharolobus solfataricus
16.8
-
 N-acetyl-L-leucyl 4-nitroanilide pH 7.5, 80°C Saccharolobus solfataricus