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Literature summary for 3.4.18.1 extracted from

  • Jevnikar, Z.; Obermajer, N.; Pecar-Fonovi?, U.; Karaoglanovic-Carmona, A.; Kos, J.
    Cathepsin X cleaves the beta2 cytoplasmic tail of LFA-1 inducing the intermediate affinity form of LFA-1 and alpha-actinin-1 binding (2009), Eur. J. Immunol., 39, 3217-3227.
    View publication on PubMed
Search for more literature for 3.4.18.1

Cloned(Commentary)

Cloned (Comment) Organism
cathepsin X stable expression in Jurkat T cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information cathepsin X overexpression reduces LFA-1 clustering and induces an intermediate affinity LFA-1 conformation that is known to associate with alpha-actinin-1. Migration of primary T cell is reduced by cathepsin X-specific siRNA silencing Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information cathepsin X inhibition by mAb 2F12. Migration of primary T cell is reduced by mAb 2F12 by 0.27fold Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
lymphocyte function associated antigen-1 + H2O Homo sapiens cathepsin X cleaves the beta2 cytoplasmic tail of LFA-1 inducing the intermediate affinity form of LFA-1 and alpha-actinin-1 binding. Cleavage by cathepsin X of the amino acid residues S769, E768 and A767 from the C-terminal of the b2 cytoplasmic tail of LFA-1 promotes binding of the actin-binding protein a-actinin-1 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
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-
-

Source Tissue

Source Tissue Comment Organism Textmining
T-cell
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 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Abz-Phe-Glu-Lys(Dnp)-OH + H2O
-
 Homo sapiens ?
-
 ?
lymphocyte function associated antigen-1 + H2O cathepsin X cleaves the beta2 cytoplasmic tail of LFA-1 inducing the intermediate affinity form of LFA-1 and alpha-actinin-1 binding. Cleavage by cathepsin X of the amino acid residues S769, E768 and A767 from the C-terminal of the b2 cytoplasmic tail of LFA-1 promotes binding of the actin-binding protein a-actinin-1 Homo sapiens ?
-
 ?
lymphocyte function associated antigen-1 + H2O cathepsin X cleaves the amino acid residues S769, E768 and A767 from the C-terminal of LFA-1 Homo sapiens ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 assay at Homo sapiens

General Information

General Information Comment Organism
physiological function cathepsin X, a cysteine protease, regulates T-cell migration by interaction with lymphocyte function associated antigen-1, LFA-1. Gradual cleavage of LFA-1 by cathepsin X enables the transition between intermediate and high affinity LFA-1, an event that is crucial for effective T-cell migration, overview Homo sapiens