Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression analysis, expression of GST-tagged enzyme in Escherichia coli strain BL21 | Cyprinus carpio |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | surrounding the yolk granules, in oocytes | Cyprinus carpio | 5737 | - |
external encapsulating structure | dislocation of CTPZ from the cortical granules | Cyprinus carpio | 30312 | - |
plasma membrane | dislocation of CTPZ from the cortical granules | Cyprinus carpio | 5886 | - |
yolk granule | association with, after fertilization | Cyprinus carpio | 42718 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
x * 28000, recombinant enzyme, SDS-PAGE | Cyprinus carpio |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cyprinus carpio | Q58HG7 | CTPZ; gene CTPZ | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme contains one potential N-glycosylation site | Cyprinus carpio |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged enzyme from Escherichia coli strain BL21 by glutathione affinity and anion exchange chromatography | Cyprinus carpio |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
Release of C-terminal amino acid residues with broad specificity, but lacks action on C-terminal proline. Shows weak endopeptidase activity | the catalytic triad is formed by Cys84, His233, and Asn254 | Cyprinus carpio |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
granulosa cell | in oocytes | Cyprinus carpio | - |
additional information | wide tissue distribution of enzyme expression | Cyprinus carpio | - |
oocyte | enzyme mRNA accumulation during maturation | Cyprinus carpio | - |
Subunits | Comment | Organism |
---|---|---|
? | x * 28000, recombinant enzyme, SDS-PAGE | Cyprinus carpio |
Synonyms | Comment | Organism |
---|---|---|
cathepsin Z | - |
Cyprinus carpio |
CTPZ | - |
Cyprinus carpio |