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Literature summary for 3.4.17.B4 extracted from

  • Lee, H.S.; Kim, Y.J.; Bae, S.S.; Jeon, J.H.; Lim, J.K.; Kang, S.G.; Lee, J.H.
    Overexpression and characterization of a carboxypeptidase from the hyperthermophilic archaeon Thermococcus sp. NA1 (2006), Biosci. Biotechnol. Biochem., 70, 1140-1147.
    View publication on PubMed
Search for more literature for 3.4.17.B4

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Thermococcus onnurineus

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 0.2 mM, about 90% loss of activity, the inhibitory effect is not overcome by the presence of Co2+ Thermococcus onnurineus
EDTA 1 mM, complete inactivation Thermococcus onnurineus
Fe2+ 0.2 mM, about 70% loss of activity, the inhibitory effect is not overcome by the presence of Co2+ Thermococcus onnurineus
Ni2+ 0.2 mM, about 75% loss of activity, the inhibitory effect is not overcome by the presence of Co2+ Thermococcus onnurineus
Zn2+ 0.2 mM, about 90% loss of activity, the inhibitory effect is not overcome by the presence of Co2+ Thermococcus onnurineus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4
-
 N-benzyloxycarbonyl-Ala-Arg pH 6.5, 70-80°C Thermococcus onnurineus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 0.2 mM, stimulates activity, can not be replaced by other divalent metal ions, no activation by Zn2+ Thermococcus onnurineus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000
-
 2 * 61000, SDS-PAGE Thermococcus onnurineus
135000
-
 gel filtration Thermococcus onnurineus

Organic Solvent Stability

Organic Solvent Comment Organism
Glycerol 12.5%, does not protect from inactivation at either temperature Thermococcus onnurineus

Organism

Organism UniProt Comment Textmining
Thermococcus onnurineus Q2QC89
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe + H2O
-
 Thermococcus onnurineus Ac-Asp-Arg-Val-Tyr-Ile-His-Pro + L-phenylalanine
-
 ?
Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His + H2O
-
 Thermococcus onnurineus Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe + L-histidine
-
 ?
Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu + H2O
-
 Thermococcus onnurineus Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His + L-leucine
-
 ?
Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu + H2O
-
 Thermococcus onnurineus Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu + L-leucine
-
 ?
Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val + H2O
-
 Thermococcus onnurineus Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu + L-valine
-
 ?
Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val-Tyr + H2O
-
 Thermococcus onnurineus Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val + L-tyrosine
-
 ?
Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val-Tyr-Ser + H2O release of up to seven residues from the C-terminus Thermococcus onnurineus Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val-Tyr + L-serine
-
 ?
additional information no activity with N-benzyloxycarbonyl-Ala-Asp, N-benzyloxycarbonyl-Ala-Asn, N-benzyloxycarbonyl-Ala-Pro, N-benzyloxycarbonyl-Ala-His Thermococcus onnurineus ?
-
 ?
N-benzyloxycarbonyl-Ala-Ala + H2O
-
 Thermococcus onnurineus N-benzyloxycarbonyl-Ala + L-alanine
-
 ?
N-benzyloxycarbonyl-Ala-Arg + H2O compared to the activity of Pyrococcus furiosus carboxypeptidase the catalytic efficiency is of Thermococcus onnurineus carboxypeptidase is 80-fold lower Thermococcus onnurineus N-benzyloxycarbonyl-Ala + L-arginine
-
 ?
N-benzyloxycarbonyl-Ala-Leu + H2O
-
 Thermococcus onnurineus N-benzyloxycarbonyl-Ala + L-leucine
-
 ?
N-benzyloxycarbonyl-Ala-Met + H2O
-
 Thermococcus onnurineus N-benzyloxycarbonyl-Ala + L-methionine
-
 ?
N-benzyloxycarbonyl-Ala-Tyr + H2O
-
 Thermococcus onnurineus N-benzyloxycarbonyl-Ala + L-tyrosine
-
 ?
N-benzyloxycarbonyl-Ala-Val + H2O
-
 Thermococcus onnurineus N-benzyloxycarbonyl-Ala + L-valine
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 61000, SDS-PAGE Thermococcus onnurineus

Synonyms

Synonyms Comment Organism
TNA1_CP
-
 Thermococcus onnurineus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70 80 substrate: N-benzyloxycarbonyl-Ala-Arg Thermococcus onnurineus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 90 50°C: about 50% of maximal activity, 90°C: about 60% of maximal activity Thermococcus onnurineus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 pH 6.5, 0.2 mM Co2+, t1/2: 84 min, t1/2 in absence of Co2+ is 7.4 min Thermococcus onnurineus
90
-
 pH 6.5, 0.2 mM Co2+, t1/2: 8 min, t1/2 in absence of Co2+ is 5 min Thermococcus onnurineus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11.6
-
 N-benzyloxycarbonyl-Ala-Arg pH 6.5, 70-80°C Thermococcus onnurineus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 substrate: N-benzyloxycarbonyl-Ala-Arg Thermococcus onnurineus

pH Range

pH Minimum pH Maximum Comment Organism
5.3 7.5 pH 5.3: about 80% of maximal activity, pH 7.5: about 45% of maximal activity Thermococcus onnurineus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
8.3
-
 N-benzyloxycarbonyl-Ala-Arg pH 6.5, 70-80°C Thermococcus onnurineus