Literature summary for 3.4.17.8 extracted from

Smith, J.D.; Kumarasiri, M.; Zhang, W.; Hesek, D.; Lee, M.; Toth, M.; Vakulenko, S.; Fisher, J.F.; Mobashery, S.; Chen, Y.
Structural analysis of the role of Pseudomonas aeruginosa penicillin-binding protein 5 in beta-lactam resistance (2013), Antimicrob. Agents Chemother., 57, 3137-3146.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystal structure of recombinant protein, with deletion off the C-terminal anchor, at a 2.05 A resolution. Structure contains a shortened loop spanning residues 74 to 78 near the active site and two active-site residues, Ser101 and Lys203. An active-site flexibility may explain its carbapenemase activity	Pseudomonas aeruginosa

Protein Variants

Protein Variants	Comment	Organism
additional information	expression of recombinant protein as soluble version by deletion of its C-terminal membrane anchor. Under in vitro conditions, the soluble enzyme demonstrates both D,D-carboxypeptidase and expanded-spectrum beta-lactamase activities	Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
6.1	-	methyl N-acetylmuramate-L-Ala-gamma-D-Glu-meso-diaminopimelate-D-Ala-D-Ala	pH not specified in the publication, temperature not specified in the publication	Pseudomonas aeruginosa
8.7	-	Nalpha,Nepsilon-Diacetyl-L-Lys-D-Ala-D-Ala	pH not specified in the publication, temperature not specified in the publication	Pseudomonas aeruginosa

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	G3XD74	-	-
Pseudomonas aeruginosa ATCC 15692	G3XD74	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
methyl N-acetylmuramate-L-Ala-gamma-D-Glu-meso-diaminopimelate-D-Ala-D-Ala + H2O	-	Pseudomonas aeruginosa	methyl N-acetylmuramate-L-Ala-gamma-D-Glu-meso-diaminopimelate-D-Ala + D-Ala	-	?
methyl N-acetylmuramate-L-Ala-gamma-D-Glu-meso-diaminopimelate-D-Ala-D-Ala + H2O	-	Pseudomonas aeruginosa ATCC 15692	methyl N-acetylmuramate-L-Ala-gamma-D-Glu-meso-diaminopimelate-D-Ala + D-Ala	-	?
additional information	enzyme displays both D,D-carboxypeptidase and expanded-spectrum beta-lactamase activities	Pseudomonas aeruginosa	?	-	?
additional information	enzyme displays both D,D-carboxypeptidase and expanded-spectrum beta-lactamase activities	Pseudomonas aeruginosa ATCC 15692	?	-	?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O	-	Pseudomonas aeruginosa	Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-Ala	-	?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O	-	Pseudomonas aeruginosa ATCC 15692	Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-Ala	-	?

Synonyms

Synonyms	Comment	Organism
DacC	-	Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.31	-	methyl N-acetylmuramate-L-Ala-gamma-D-Glu-meso-diaminopimelate-D-Ala-D-Ala	pH not specified in the publication, temperature not specified in the publication	Pseudomonas aeruginosa
0.59	-	Nalpha,Nepsilon-Diacetyl-L-Lys-D-Ala-D-Ala	pH not specified in the publication, temperature not specified in the publication	Pseudomonas aeruginosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.055	-	methyl N-acetylmuramate-L-Ala-gamma-D-Glu-meso-diaminopimelate-D-Ala-D-Ala	pH not specified in the publication, temperature not specified in the publication	Pseudomonas aeruginosa
0.065	-	Nalpha,Nepsilon-Diacetyl-L-Lys-D-Ala-D-Ala	pH not specified in the publication, temperature not specified in the publication	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)