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Literature summary for 3.4.17.21 extracted from

  • Barinka, C.; Sacha, P.; Sklenar, J.; Man, P.; Bezouska, K.; Slusher, B.S.; Konvalinka, J.
    Identification of the N-glycosylation sites on glutamate carboxypeptidase II necessary for proteolytic activity (2004), Protein Sci., 13, 1627-1635.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.17.21

Cloned(Commentary)

Cloned (Comment) Organism
stable expression of wild-type and mutant enzymes in S2 cells, secretion of the enzyme, which is prevented by blocking the N-glycosylation Homo sapiens

Protein Variants

Protein Variants Comment Organism
N121A site-directed mutagenesis of an N-glycosylation site, nearly inactive enzyme Homo sapiens
N140A site-directed mutagenesis of an N-glycosylation site, nearly inactive enzyme Homo sapiens
N153A site-directed mutagenesis of an N-glycosylation site, nearly inactive enzyme Homo sapiens
N195A site-directed mutagenesis of an N-glycosylation site, nearly inactive enzyme Homo sapiens
N336A site-directed mutagenesis of an N-glycosylation site leads to 45% reduced activity compared to the wild-type enzyme Homo sapiens
N476A site-directed mutagenesis of an N-glycosylation site leads to 70% reduced activity compared to the wild-type enzyme Homo sapiens
N51A site-directed mutagenesis of an N-glycosylation site leads to 75% reduced activity compared to the wild-type enzyme Homo sapiens
N638A site-directed mutagenesis of an N-glycosylation site, nearly inactive enzyme Homo sapiens
N76A site-directed mutagenesis of an N-glycosylation site leads to 25% reduced activity compared to the wild-type enzyme Homo sapiens
T640A site-directed mutagenesis of an N-glycosylation site, nearly inactive enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.000066
-
 N-acetyl-alpha-L-aspartyl-L-glutamate recombinant mutant N195A Homo sapiens
0.0001
-
 N-acetyl-alpha-L-aspartyl-L-glutamate recombinant mutant N121A Homo sapiens
0.000321
-
 N-acetyl-alpha-L-aspartyl-L-glutamate recombinant wild-type enzyme Homo sapiens
0.000334
-
 N-acetyl-alpha-L-aspartyl-L-glutamate recombinant mutant N638A Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane type II membrane protein Homo sapiens 16020
-
synaptic cleft
-
 Homo sapiens 43083
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-alpha-L-aspartyl-L-glutamate + H2O Homo sapiens the enzyme is responsible for cleavage of NAAG to yield free glutamate N-acetyl-alpha-L-aspartate + L-glutamate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein identification of the N-glycosylation sites, e.g. N121, N153, N195, N638, and N140, the enzyme is heavily glycosylated Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma-glutamates T640 is involved in catalysis, the N-glycosylation status is important for actalytic activity Homo sapiens
a

Source Tissue

Source Tissue Comment Organism Textmining
central nervous system
-
 Homo sapiens
-
kidney
-
 Homo sapiens
-
peripheral nervous system
-
 Homo sapiens
-
prostate gland prostate form of the enzyme is termed prostate-specific membrane antigen Homo sapiens
-
small intestine
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-alpha-L-aspartyl-L-glutamate + H2O the enzyme is responsible for cleavage of NAAG to yield free glutamate Homo sapiens N-acetyl-alpha-L-aspartate + L-glutamate
-
 ?
N-acetyl-alpha-L-aspartyl-L-glutamate + H2O i.e. NAAG Homo sapiens N-acetyl-alpha-L-aspartate + L-glutamate
-
 ?

Synonyms

Synonyms Comment Organism
GCPII
-
 Homo sapiens
N-Acetylated alpha-linked acidic dipeptidase
-
 Homo sapiens
NAAG-hydrolyzing activity
-
 Homo sapiens
NAALADase
-
 Homo sapiens
Prostate-specific membrane antigen
-
 Homo sapiens
PSMA
-
 Homo sapiens