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Literature summary for 3.4.17.21 extracted from
- Crystal structure of prostate-specific membrane antigen, a tumor marker and peptidase (2005), Proc. Natl. Acad. Sci. USA, 102, 5981-5986.
Application
| Application | Comment | Organism |
|---|---|---|
| diagnostics | prostate-specific membrane antigen is a tumor marker | Homo sapiens |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloning and expression of the His6-tagged extracellular portion of the enzyme in High5 cells using the baculovirus transfection system, secretion of the glycosylated extracellular domain | Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| purified recombinant His6-tagged extracellular portion, which contains bound Zn2+, hanging drop vapour diffusion method, 0.0008 ml of 10 mg/ml protein in 20 mM Tris, pH 7.5, is mixed with an equal volume of reservoir solution containing 18% PEG 3350, 0.2 M sodium thiocyanate, 4°C, cryoprotection by 20% glycerol, X-ray diffrcation structure determination and analysis at 3.5 A resolution | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Homo sapiens | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | the enzyme contains a binuclear Zn2+ binding site, involved in the reaction mechanism | Homo sapiens |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-alpha-L-aspartyl-L-glutamate + H2O | Homo sapiens | - |
N-acetyl-alpha-L-aspartate + L-glutamate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q04609 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Homo sapiens |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged extracellular portion of the enzyme from High5 by nickel affinity chromatography and gel filtration | Homo sapiens |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| release of an unsubstituted, C-terminal glutamyl residue, typically from Ac-Asp-Glu or folylpoly-gamma-glutamates | active site structure, substrate binding involving Asp210, and catalytic reaction mechanism involving Glu424 and the Zn2+ ions, the substrate binding cavity involves residues Arg463, Arg534, Arg536, stabilized by Glu457, Asp465, and Ser454 | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| prostate cancer cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N-acetyl-alpha-L-aspartyl-L-glutamate + H2O | - |
Homo sapiens | N-acetyl-alpha-L-aspartate + L-glutamate | - |
? | |
| N-acetyl-alpha-L-aspartyl-L-glutamate + H2O | i.e. NAAG | Homo sapiens | N-acetyl-alpha-L-aspartate + L-glutamate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GCPII | - |
Homo sapiens |
| N-acetyl-alpha-linked acidic dipeptidase I | - |
Homo sapiens |
| Naaladase I | - |
Homo sapiens |
| NLD I | - |
Homo sapiens |
| Prostate-specific membrane antigen | - |
Homo sapiens |
| PSMA | - |
Homo sapiens |
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Release 2023.1 (January 2023)
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