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Literature summary for 3.4.17.20 extracted from

  • Bouma, B.N.; Marx, P.F.; Mosnier, L.O.; Meijers, J.C.
    Thrombin-activatable fibrinolysis inhibitor (TAFI, plasma procarboxypeptidase B, procarboxypeptidase R, procarboxypeptidase U) (2001), Thromb. Res., 101, 329-354.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information activation of TAFI by thrombin is an inefficient process, thrombomodulin stimulates the activation 1250fold. Activation by plasmin is stimulated by glycosaminoglycan Homo sapiens

Application

Application Comment Organism
medicine agents that improve the efficiency of TAFI to downregulate fibrinolysis might become available for the treatment of bleeding disorders such as hemophilias A and B Homo sapiens

General Stability

General Stability Organism
epsilon-aminocaproic acid, heparin or guanidinoethyl-mercaptosuccinic acid stabilize Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline
-
Homo sapiens
2-Bromo-4-methylbutan-1,4-olide
-
Homo sapiens
2-mercaptoethanol
-
Homo sapiens
dithiothreitol
-
Homo sapiens
EDTA
-
Homo sapiens
epsilon-aminocaproic acid
-
Homo sapiens
Guanidinoethylmercaptosuccinic acid
-
Homo sapiens
p-chloromercuribenzoate
-
Homo sapiens
peptide inhibitor from Hirudo medicinalis
-
Homo sapiens
potato carboxypeptidase inhibitor
-
Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zinc zinc metalloenzyme Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
fibrin + H2O Homo sapiens
-
Lys + ?
-
?

Organism

Organism UniProt Comment Textmining
Canis lupus familiaris
-
-
-
Cavia porcellus
-
-
-
Homo sapiens
-
polymorphism: A/T147, A/G152, A/G438, C/T530, C/T1053, T/G1102, A/G690, T/C1925
-
Mus musculus
-
-
-
Oryctolagus cuniculus
-
-
-
Papio sp.
-
-
-
Rattus norvegicus
-
-
-
Sus scrofa
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the activation peptide contains four potential N-linked glycosylation sites at Asn22, Asn51, Asn63 and Asn96. The 36000 catalytic unit of 309 amino acids is not glycosylated. The glycosylation of the activation peptide may act to stabilize and increase the half-life of circulating TAFI Homo sapiens
proteolytic modification the proenzyme has a MW of 55000 Da (401 aa), the enzyme has a MW of 35999 Da (309 aa), activated at proteolytis at Arg92 Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
blood plasma
-
Cavia porcellus
-
blood plasma
-
Mus musculus
-
blood plasma
-
Rattus norvegicus
-
blood plasma
-
Sus scrofa
-
blood plasma
-
Oryctolagus cuniculus
-
blood plasma
-
Canis lupus familiaris
-
blood plasma
-
Papio sp.
-
blood plasma TAFI circulates at a concentration of 0.004-0.015 mg/ml Homo sapiens
-
liver synthesized in Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
fibrin + H2O
-
Homo sapiens Lys + ?
-
?

Synonyms

Synonyms Comment Organism
TAFI zymogen Cavia porcellus
TAFI zymogen Mus musculus
TAFI zymogen Homo sapiens
TAFI zymogen Rattus norvegicus
TAFI zymogen Sus scrofa
TAFI zymogen Oryctolagus cuniculus
TAFI zymogen Canis lupus familiaris
TAFI zymogen Papio sp.
TAFIa active enzyme Cavia porcellus
TAFIa active enzyme Mus musculus
TAFIa active enzyme Homo sapiens
TAFIa active enzyme Rattus norvegicus
TAFIa active enzyme Sus scrofa
TAFIa active enzyme Oryctolagus cuniculus
TAFIa active enzyme Canis lupus familiaris
TAFIa active enzyme Papio sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
22
-
half-life: several hours Homo sapiens
30
-
half-life: 45 min Homo sapiens
37
-
half-life: 10 min Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.7
-
-
Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0000004
-
potato carboxypeptidase inhibitor
-
Homo sapiens
0.018
-
Guanidinoethylmercaptosuccinic acid
-
Homo sapiens
0.8
-
epsilon-aminocaproic acid
-
Homo sapiens

pI Value

Organism Comment pI Value Maximum pI Value
Homo sapiens the attachment of carbohydrates to the protein constitutes approximately 20% of the total mass
-
5