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Literature summary for 3.4.17.2 extracted from
- Isolation and characteristics of carboxypeptidase B from zebra blenny (Salaria basilisca) viscera (2014), J. Aquatic Food Product Technol., 23, 208-220.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | inhibits activity | Salaria basilisca |  |
| EDTA | - |
Salaria basilisca | |
| Hg2+ | inhibits activity | Salaria basilisca | |
| Mn2+ | inhibits activity | Salaria basilisca | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.32 | - |
hippuryl-L-Arg | pH 8, 60°C | Salaria basilisca | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activates enzyme | Salaria basilisca | |
| Zn2+ | activates enzyme | Salaria basilisca | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34500 | - |
SDS-PAGE | Salaria basilisca |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salaria basilisca | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using ammonium sulfate precipitation and gel filtration | Salaria basilisca |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Hippuryl-L-Arg + H2O | - |
Salaria basilisca | Hippuric acid + L-Arg | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| carboxypeptidase B | - |
Salaria basilisca |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Salaria basilisca |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
the enzyme is unstable above 50°C | Salaria basilisca |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 36.23 | - |
hippuryl-L-Arg | pH 8, 60°C | Salaria basilisca | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Salaria basilisca |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
the enzyme is unstable below pH 5 | Salaria basilisca |
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