BRENDA - Enzyme Database show
show all sequences of 3.4.17.15

Carboxypeptidases A1 and A2 from the perfusate of rat mesenteric arterial bed differentially process angiotensin peptides

Pereira, H.; Souza, L.; Costa-Neto, C.; Salgado, M.; Oliveira, E.; Peptides 33, 67-76 (2012)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
1 mM, complete inhibition
Rattus norvegicus
potato carboxypeptidase inhibitor
10 microM, complete inhibition
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.191
-
angiotensin II
pH 8.1, 37°C
Rattus norvegicus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Purification (Commentary)
Commentary
Organism
from mesenteric arterial bed perfusate
Rattus norvegicus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
artery
mesenteric arterial bed, enzyme is identical with its pancreatic counterpart
Rattus norvegicus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
angiotensin II + H2O
-
732609
Rattus norvegicus
angiotensin-(1-7) + L-Phe
-
-
-
?
angiotensin-(1-12) + H2O
-
732609
Rattus norvegicus
angiotensin I + ?
conversion of Ang-(1-12) to Ang I proceeds through stepwise cleavage of C-terminal Tyr and Leu residues
-
-
?
benzyloxycarbonyl-Val-Phe + H2O
-
732609
Rattus norvegicus
benzyloxycarbonyl-Val + L-Phe
-
-
-
?
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
1 mM, complete inhibition
Rattus norvegicus
potato carboxypeptidase inhibitor
10 microM, complete inhibition
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.191
-
angiotensin II
pH 8.1, 37°C
Rattus norvegicus
Purification (Commentary) (protein specific)
Commentary
Organism
from mesenteric arterial bed perfusate
Rattus norvegicus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
artery
mesenteric arterial bed, enzyme is identical with its pancreatic counterpart
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
angiotensin II + H2O
-
732609
Rattus norvegicus
angiotensin-(1-7) + L-Phe
-
-
-
?
angiotensin-(1-12) + H2O
-
732609
Rattus norvegicus
angiotensin I + ?
conversion of Ang-(1-12) to Ang I proceeds through stepwise cleavage of C-terminal Tyr and Leu residues
-
-
?
benzyloxycarbonyl-Val-Phe + H2O
-
732609
Rattus norvegicus
benzyloxycarbonyl-Val + L-Phe
-
-
-
?
Other publictions for EC 3.4.17.15
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
732350
Jitonnom
Comparative study on activatio ...
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298-303
2012
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732609
Pereira
Carboxypeptidases A1 and A2 fr ...
Rattus norvegicus
Peptides
33
67-76
2012
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668948
Liu
Cloning and characterization o ...
Mayetiola destructor
Insect Biochem. Mol. Biol.
36
665-673
2006
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668310
Srivastava
Molecular cloning and cDNA seq ...
Paralichthys olivaceus
Comp. Biochem. Physiol. B
135
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2003
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Bentley
The imprinted region on human ...
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670816
Jimenez
NMR solution structure of the ...
Homo sapiens
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12
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Latexin, a carboxypeptidase A ...
Rattus norvegicus
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