Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression of the pro-CPA2 in Saccharomyces cerevisiae | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
methotrexate-alpha-(1-naphthyl)alanine | pH 7.4, 25°C, recombinant enzyme | Homo sapiens | |
0.056 | - |
methotrexate-alpha-phenylalanine | pH 7.4, 25°C, recombinant enzyme | Homo sapiens | |
0.49 | - |
hippuryl-L-phenylalanine | pH 7.4, 25°C, recombinant enzyme | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
x * 34000, recombinant enzyme, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | the proCPA2 is activated by cleavage by trypsin | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant pro-CPA2 from Saccharomyces cerevisiae by hydrophobic interaction and anion exchange chromatography to homogeneity | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
catalytic efficency of the purified recombinant enzyme | Homo sapiens |
8.7 | - |
purified recombinant enzyme, substrate hippuryl-L-phenylalanine | Homo sapiens |
2800 | - |
purified recombinant enzyme, substrate hippuryl-L-phenyllactate | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hippuryl-DL-phenyllactate + H2O | preferred substrate | Homo sapiens | hippuric acid + DL-phenyllactate | - |
? | |
hippuryl-L-phenylalanine + H2O | - |
Homo sapiens | hippuric acid + L-phenylalanine | - |
? | |
methotrexate-alpha-(1-naphthyl)alanine + H2O | - |
Homo sapiens | methotrexate + (1-naphthyl)alanine | - |
? | |
methotrexate-alpha-phenylalanine + H2O | - |
Homo sapiens | methotrexate + phenylalanine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34000, recombinant enzyme, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
CPA2 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
half-life of purified recombinant CPA2 is 24 min, and 8fold longer than for CPA1 | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.1 | - |
methotrexate-alpha-phenylalanine | pH 7.4, 25°C, recombinant enzyme | Homo sapiens | |
9.3 | - |
hippuryl-L-phenylalanine | pH 7.4, 25°C, recombinant enzyme | Homo sapiens | |
22 | - |
methotrexate-alpha-(1-naphthyl)alanine | pH 7.4, 25°C, recombinant enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |