Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | - |
Priestia megaterium | |
Mg2+ | optimal concentration for stimulation: 10 mM, inhibition above | Priestia megaterium | |
NEM | weak | Priestia megaterium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | can replace Mg2+ in stimulation | Priestia megaterium | |
Mg2+ | stimulates, optimal concentration: 10 mM, inhibition above | Priestia megaterium | |
Mn2+ | can replace Mg2+ in stimulation | Priestia megaterium | |
additional information | no stimulation by Zn2+, Cu2+ | Priestia megaterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
gel filtration | Priestia megaterium |
60000 | - |
1 * 60000, Bacillus megaterium, gel filtration | Priestia megaterium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Priestia megaterium | incorporation of diaminopimelate into cell walls | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Priestia megaterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | incorporation of diaminopimelate into cell walls | Priestia megaterium | ? | - |
? | |
UDP-N-acetylmuramyl-Ala-D-Glu-Dap-D-Ala + H2O | enzyme can also replace the terminal D-alanine with a variety of amino acids with D-asymmetric centers for transpeptidation | Priestia megaterium | UDP-N-acetylmuramyl-Ala-D-Glu-Dap + D-Ala | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60000, Bacillus megaterium, gel filtration | Priestia megaterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Priestia megaterium |
30 | 60 | incorporation of diaminopimelate into cell walls | Priestia megaterium |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 60 | 10°C: 27% of activity maximum, 60°C: 99% of activity maximum | Priestia megaterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.2 | - |
Priestia megaterium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.8 | 9.5 | 5.8: about 40% of activity maximum, 9.5: about 30% of activity maximum | Priestia megaterium |