Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, genetic structure, localization on chromosome 12q13-q15, expression in insect cells via baculovirus transfection | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,10-phenanthroline | - |
Homo sapiens | |
Cadmium acetate | - |
Homo sapiens | |
DL-2-mercaptomethyl-3-guanidinoethylthiopropanoic acid | inhibits best at acidic pH | Homo sapiens | |
Guanidinoethylmercaptosuccinic acid | - |
Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell surface | - |
Homo sapiens | 9986 | - |
cell surface | - |
Canis lupus familiaris | 9986 | - |
extracellular | - |
Homo sapiens | - |
- |
extracellular | - |
Canis lupus familiaris | - |
- |
membrane | bound | Canis lupus familiaris | 16020 | - |
plasma membrane | anchored via glycosylphosphatidylinositol | Homo sapiens | 5886 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activates, best at acidic pH | Homo sapiens | |
additional information | the enzyme is a metallopeptidase | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47600 | - |
1 * 62000, glycosylated enzyme, SDS-PAGE, 1 * 47600, deglycosylated enzyme, SDS-PAGE | Homo sapiens |
62000 | - |
1 * 62000, glycosylated enzyme, SDS-PAGE, 1 * 47600, deglycosylated enzyme, SDS-PAGE | Homo sapiens |
73000 | - |
gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
epidermal growth factor + H2O | Homo sapiens | - |
dea-Arg53-epidermal growth factor + arginine | - |
? | |
additional information | Homo sapiens | the enzyme controls the activity of kinins in the kidney, is involved in inflammation, and participates in the metabolism of growth factors that contain C-terminal Arg or Lys residues, e.g. the epidermal growth factor, the nerve growth factor, the hypatocyte growth factor, the macrophage-stimulating protein, or erythropoietin | ? | - |
? | |
additional information | Canis lupus familiaris | the enzyme may be responsible for control of kinins and epidermal growth factor as well as of EGF-like peptides | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Canis lupus familiaris | - |
- |
- |
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme contains 6 potential glycosylation sites and 23% carbohydrate content | Homo sapiens |
Purification (Comment) | Organism |
---|---|
native enzyme from placental microvillus membranes by solubilization through either phosphatidylinositol-specific phospholipase C or CHAPS, ion exchange and arginine affinity chromatography, and gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
amniotic fluid | - |
Canis lupus familiaris | - |
blood vessel | - |
Homo sapiens | - |
brain | - |
Homo sapiens | - |
intestine | - |
Homo sapiens | - |
kidney | - |
Homo sapiens | - |
kidney | synthesized and released in distal tubules | Canis lupus familiaris | - |
lung | - |
Homo sapiens | - |
MDCK cell | Madnine-Darby canine kidney cells, high expression level | Canis lupus familiaris | - |
nerve | peripheral | Homo sapiens | - |
placenta | microvillus | Homo sapiens | - |
urine | - |
Canis lupus familiaris | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
epidermal growth factor + H2O | - |
Homo sapiens | dea-Arg53-epidermal growth factor + arginine | - |
? | |
additional information | the enzyme controls the activity of kinins in the kidney, is involved in inflammation, and participates in the metabolism of growth factors that contain C-terminal Arg or Lys residues, e.g. the epidermal growth factor, the nerve growth factor, the hypatocyte growth factor, the macrophage-stimulating protein, or erythropoietin | Homo sapiens | ? | - |
? | |
additional information | the enzyme may be responsible for control of kinins and epidermal growth factor as well as of EGF-like peptides | Canis lupus familiaris | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 62000, glycosylated enzyme, SDS-PAGE, 1 * 47600, deglycosylated enzyme, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
CPM | - |
Homo sapiens |
CPM | - |
Canis lupus familiaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
neutral pH-optimum | Homo sapiens |