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Literature summary for 3.4.17.11 extracted from

  • Sherwood, R.F.; Melton, R.G.; Alwan, S.M.; Hughes, P.
    Purification and properties of carboxypeptidase G2 from Pseudomonas sp. strain RS-16. Use of a novel triazine dye affinity method (1985), Eur. J. Biochem., 148, 447-453.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.004
-
 folate
-
 Pseudomonas sp.
0.008
-
 methotrexate
-
 Pseudomonas sp.
0.034
-
 5-methyltetrahydrofolate
-
 Pseudomonas sp.
0.12
-
 5-formyltetrahydrofolate
-
 Pseudomonas sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains 4 atoms of zinc per enzyme molecule, which are required for full activity Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41800
-
 2 * 41800, SDS-PAGE Pseudomonas sp.
83000
-
 gel filtration Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
large scale Pseudomonas sp.
strain RS-16 Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
215
-
-
 Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-formyltetrahydrofolic acid + H2O
-
 Pseudomonas sp. 5-formylpteroate + Glu
-
 ?
5-methyltetrahydrofolate + H2O
-
 Pseudomonas sp. 5-methylpteroate + Glu
-
 ?
folic acid + H2O
-
 Pseudomonas sp. pteroic acid + glutamic acid
-
 ?
methotrexate + H2O
-
 Pseudomonas sp. 4-amino-4-deoxy-N10-methylpteroic acid + L-glutamate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 41800, SDS-PAGE Pseudomonas sp.