General Stability | Organism |
---|---|
bovine albumin stabilizes against heat inactivation | Flavobacterium sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Flavobacterium sp. | |
citrate | - |
Flavobacterium sp. | |
CoCl2 | - |
Flavobacterium sp. | |
DTT | - |
Flavobacterium sp. | |
EDTA | Ca2+, Co2+, Mg2+ and Zn2+ reverse inhibition | Flavobacterium sp. | |
L-Glutamic acid | weak | Flavobacterium sp. | |
potassium ascorbate | 1 mM: harmless, 3 mM: strong inhibition | Flavobacterium sp. | |
Pteroic acid | - |
Flavobacterium sp. | |
ZnCl2 | - |
Flavobacterium sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | weak activator in absence of EDTA | Flavobacterium sp. | |
Zn2+ | weak activator in absence of EDTA | Flavobacterium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
gel filtration | Flavobacterium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Flavobacterium sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Flavobacterium sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
39.43 | - |
- |
Flavobacterium sp. |
Storage Stability | Organism |
---|---|
-20°C, stable for 1 year | Flavobacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dihydroxypteroyl-L-glutamic acid + H2O | - |
Flavobacterium sp. | 2,4-dihydroxypteroate + L-Glu | - |
? | |
4-aminopteroylglutamic acid + H2O | i.e. aminopterin | Flavobacterium sp. | 4-aminopteroate + Glu | - |
? | |
5-formyltetrahydrofolic acid + H2O | i.e. leucovorin | Flavobacterium sp. | 5-formylpteroate + Glu | - |
? | |
5-methyltetrahydrofolate + H2O | weak | Flavobacterium sp. | 5-methylpteroate + Glu | - |
? | |
folic acid + H2O | - |
Flavobacterium sp. | pteroic acid + glutamic acid | - |
? | |
methotrexate + H2O | - |
Flavobacterium sp. | 4-amino-4-deoxy-N10-methylpteroic acid + L-glutamate | - |
? | |
additional information | releases the carboxy-terminal glutamyl moiety of derivatives of pteroyl-mono-L-glutamic acid | Flavobacterium sp. | ? | - |
? | |
additional information | substituents on N5 of the pteridine ring decrease the velocity of hydrolysis | Flavobacterium sp. | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
with methotrexate and 5-methyltetrahydrofolate as substrates, the reaction proceeds equally well between 30°C and 42°C | Flavobacterium sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
half-life: 4 h | Flavobacterium sp. |
25 | - |
half-life: 90 min | Flavobacterium sp. |
37 | - |
half-life: 45 min | Flavobacterium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 8 | hydrolysis of 5-methyltetrahydrofolate, phosphate buffer | Flavobacterium sp. |
6.5 | 6.8 | hydrolysis of leucoverin, phosphate buffer | Flavobacterium sp. |
7 | 7.5 | - |
Flavobacterium sp. |
7 | 8 | hydrolysis of 5-methyltetrahydrofolate, Tris buffer | Flavobacterium sp. |
7.2 | - |
hydrolysis of methotrexate, folic acid | Flavobacterium sp. |
7.5 | 8 | hydrolysis of leucoverin, Tris buffer | Flavobacterium sp. |