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Literature summary for 3.4.17.11 extracted from
- Purification and properties of carboxypeptidase G 1 (1971), J. Biol. Chem., 246, 7207-7213.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-Aminopteroylaspartic acid | hydrolysis of 4-amino-N10-methylpteroylglutamic acid | Pseudomonas stutzeri |  |
| L-aspartic acid | weak inhibition of hydrolysis of 4-amino-N10-methylpteroylglutamic acid | Pseudomonas stutzeri | |
| L-Glutamic acid | hydrolysis of 4-amino-N10-methylpteroylglutamic acid, no inhibition by D-glutamic acid | Pseudomonas stutzeri | |
| N-Benzyloxycarbonyl glutamic acid | hydrolysis of 4-amino-N10-methylpteroylglutamic acid | Pseudomonas stutzeri | |
| N-Benzyloxycarbonylaspartic acid | hydrolysis of 4-amino-N10-methylpteroylglutamic acid | Pseudomonas stutzeri | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0011 | - |
pteroylglutamic acid | - |
Pseudomonas stutzeri | |
| 0.0039 | - |
methotrexate | - |
Pseudomonas stutzeri | |
| 0.0083 | - |
aminopterin | - |
Pseudomonas stutzeri | |
| 0.0129 | - |
5-methyltetrahydrofolic acid | - |
Pseudomonas stutzeri | |
| 0.0181 | - |
5-formyltetrahydrofolic acid | - |
Pseudomonas stutzeri | |
| 0.104 | - |
4-aminopteroylaspartate | - |
Pseudomonas stutzeri | |
| 0.58 | - |
4-amino-N10-methylpteroylaspartate | - |
Pseudomonas stutzeri | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | is the only heavy metal ion capable of restoring full activity when the endogenous metal is removed by incubation with the chelating resin | Pseudomonas stutzeri | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
2 * 46000, SDS-PAGE | Pseudomonas stutzeri |
| 92000 | - |
gel filtration | Pseudomonas stutzeri |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas stutzeri | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pseudomonas stutzeri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 725 | - |
- |
Pseudomonas stutzeri |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 0.01 M Tris-chloride buffer, pH 7.3, 0.01 mM ZnCl2, stable for several months | Pseudomonas stutzeri |
| 4°C, 0.01 M Tris-chloride buffer, pH 7.3, 0.01 mM ZnCl2, stable for 2 to 3 weeks | Pseudomonas stutzeri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-amino-N10-methylpteroylaspartic acid + H2O | - |
Pseudomonas stutzeri | 4-amino-N10-methylpteroate + Asp | - |
? | |
| 4-aminobenzoyl-L-glutamic acid + H2O | - |
Pseudomonas stutzeri | 4-aminobenzoate + glutamate | - |
? | |
| 4-aminopteroylaspartic acid + H2O | - |
Pseudomonas stutzeri | 4-aminopteroate + Asp | - |
? | |
| 4-aminopteroylglutamic acid + H2O | i.e. aminopterin | Pseudomonas stutzeri | 4-aminopteroate + Glu | - |
? | |
| 5-formyltetrahydrofolic acid + H2O | i.e. leucovorin | Pseudomonas stutzeri | 5-formylpteroate + Glu | - |
? | |
| 5-methyltetrahydrofolate + H2O | - |
Pseudomonas stutzeri | 5-methylpteroate + Glu | - |
? | |
| alpha-L-glutamyl-L-glutamic acid + H2O | - |
Pseudomonas stutzeri | L-Glu + L-Glu | - |
? | |
| aminopterin + H2O | - |
Pseudomonas stutzeri | ? | - |
? | |
| benzoyl-L-glutamic acid + H2O | - |
Pseudomonas stutzeri | benzoate + glutamate | - |
? | |
| dihydrofolic acid + H2O | - |
Pseudomonas stutzeri | dihydropteroic acid + Glu | - |
? | |
| folic acid + H2O | i.e. pteroyl-L-glutamic acid | Pseudomonas stutzeri | pteroic acid + glutamic acid | - |
r | |
| folic acid + H2O | equilibrium lies far towards hydrolysis | Pseudomonas stutzeri | pteroic acid + glutamic acid | - |
r | |
| folic acid + H2O | no activity with pteroyl-D-glutamic acid | Pseudomonas stutzeri | pteroic acid + glutamic acid | - |
r | |
| gamma-L-glutamyl-L-glutamic acid + H2O | - |
Pseudomonas stutzeri | L-Glu + L-Glu | - |
? | |
| glycyl-L-glutamic acid + H2O | - |
Pseudomonas stutzeri | glycine + glutamate | - |
? | |
| L-tyrosyl-L-glutamic acid + H2O | - |
Pseudomonas stutzeri | tyrosine + glutamate | - |
? | |
| methotrexate + H2O | i.e. amethopterin | Pseudomonas stutzeri | 4-amino-4-deoxy-N10-methylpteroic acid + L-glutamate | - |
? | |
| additional information | hydrolyzes the COOH-terminal glutamate of oligopeptides and N-benzyloxycarbonyl glutamates | Pseudomonas stutzeri | ? | - |
? | |
| additional information | lesser activity against aspartate COOH-terminal peptide linkages | Pseudomonas stutzeri | ? | - |
? | |
| N-benzyloxycarbonyl-L-aspartic acid + H2O | - |
Pseudomonas stutzeri | benzyl carbonate + L-Asp | - |
? | |
| N-benzyloxycarbonyl-L-glutamic acid + H2O | no activity with D-glutamic acid | Pseudomonas stutzeri | benzyl carbonate + L-Glu | - |
? | |
| N-benzyloxycarbonyl-L-glutamine + H2O | - |
Pseudomonas stutzeri | benzyl carbonate + L-Gln | - |
? | |
| pteroyl-gamma,gamma-triglutamate + H2O | - |
Pseudomonas stutzeri | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 46000, SDS-PAGE | Pseudomonas stutzeri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | 7.3 | - |
Pseudomonas stutzeri |
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