Crystallization (Comment) | Organism |
---|---|
complex of enzyme with the inhibitor (S)-N-sulfamoylphenylalaninecrystals are grown by the microdialysis method by equilibrating the protein-inhibitor complex in a solution of 1.2 M LiCl and 20 mM Tris-HCl buffer (pH 7.5) against a reservoir containing 0.2 M LiCl and 20 mM Tris-HCl buffer (pH 7.5) | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(R)-2-benzyl-3-(N-sulfamoyl)aminopropanoic acid | - |
Bos taurus | |
(R)-N-sulfamoylphenylalanine | - |
Bos taurus | |
(S)-2-benzyl-3-(N-sulfamoyl)aminopropanoic acid | - |
Bos taurus | |
(S)-N-(N-benzylsulfamoyl)phenylalanine | - |
Bos taurus | |
(S)-N-(N-isopropylsulfamoyl)phenylalanine | - |
Bos taurus | |
(S)-N-(N-methylsulfamoiyl)phenylalanine | - |
Bos taurus | |
(S)-N-(N-phenylethylsulfamoyl)phenylalanine | - |
Bos taurus | |
(S)-N-methyl-N-sulfamoylphenylalanine | - |
Bos taurus | |
(S)-N-sulfamoylphenylalanine benzyl ester | pH 7.5 | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | P00730 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pancreas | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
O-(trans-p-chlorocinnamoyl)-L-phenyllactic acid + H2O | - |
Bos taurus | ? | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00065 | - |
(S)-N-sulfamoylphenylalanine benzyl ester | pH 7.5 | Bos taurus | |
0.035 | - |
(S)-N-(N-benzylsulfamoyl)phenylalanine | pH 7.5 | Bos taurus | |
0.18 | - |
(S)-N-(N-methylsulfamoiyl)phenylalanine | pH 7.5 | Bos taurus | |
0.18 | - |
(S)-N-(N-phenylethylsulfamoyl)phenylalanine | pH 7.5 | Bos taurus | |
0.47 | - |
(R)-N-sulfamoylphenylalanine | pH 7.5 | Bos taurus | |
0.61 | - |
(S)-N-(N-isopropylsulfamoyl)phenylalanine | pH 7.5 | Bos taurus | |
1.4 | - |
(R)-2-benzyl-3-(N-sulfamoyl)aminopropanoic acid | pH 7.5 | Bos taurus | |
2.9 | - |
(S)-2-benzyl-3-(N-sulfamoyl)aminopropanoic acid | pH 7.5 | Bos taurus | |
3.5 | - |
(S)-N-methyl-N-sulfamoylphenylalanine | pH 7.5 | Bos taurus |