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Literature summary for 3.4.17.1 extracted from
- Crystallization and properties of carboxypeptidase A gamma from porcine pancreas (1981), Eur. J. Biochem., 117, 383-388.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Sus scrofa |
General Stability
| General Stability | Organism |
|---|---|
| freezing destabilizes, unstable in 0.72 M (NH4)2SO4, stable in 2.1 M (NH4)2SO4 | Sus scrofa |
| unstable upon lyophilization | Sus scrofa |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,10-phenanthroline | - |
Sus scrofa |  |
| Chelating agents | - |
Sus scrofa | |
| EDTA | - |
Sus scrofa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Sus scrofa |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | metalloenzyme | Sus scrofa | |
| Zn2+ | 1 mol Zn per mol of enzyme | Sus scrofa | |
| Zn2+ | 1.01 mol Zn per mol enzyme | Sus scrofa | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28900 | - |
gel filtration | Sus scrofa |
| 38900 | - |
gel filtration | Sus scrofa |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Sus scrofa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pancreas | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 108 | - |
- |
Sus scrofa |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, sodium phosphate, pH 5.8, 17% saturation ammonium phosphate, toluene, crystals | Sus scrofa |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | amino terminal amino acid sequence analysis | Sus scrofa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Sus scrofa |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
below | Sus scrofa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
buffer-dependent | Sus scrofa |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | at 25°C | Sus scrofa |
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