Literature summary for 3.4.16.6 extracted from

Pozzuolo, S.; Breme, U.; Salis, B.; Taylor, G.; Tonon, G.; Orsini, G.
Efficient bacterial expression of fusion proteins and their selective processing by a recombinant Kex-1 protease (2008), Protein Expr. Purif., 59, 334-341.

Search for more literature for 3.4.16.6

Application

Application	Comment	Organism
biotechnology	Kex-1-C611 is proposed as a convenient biotechnological reagent for the cleavage of fusion proteins	Kluyveromyces lactis

Cloned(Commentary)

Cloned (Comment)	Organism
a truncated form of Kex-1, Kex-1-C611, is constructed comprising the amino acid residues from 104-611 and lacks the transmembrane domain	Kluyveromyces lactis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	-	Kluyveromyces lactis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
58000	-	calculated, truncated Kex-1-C611	Kluyveromyces lactis
67000	-	determined by SDS-PAGE, truncated Kex-1-C611	Kluyveromyces lactis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Kluyveromyces lactis	preferential release of a C-terminal arginine or lysine residue	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Kluyveromyces lactis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
archieved by a two-step chromatographic process using cation-exchange, SP Sepharose, followed by anion-exchange, DEAE Sepharose FF resin	Kluyveromyces lactis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	60 U/ml, purified truncated Kex-1-C611	Kluyveromyces lactis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	preferential release of a C-terminal arginine or lysine residue	Kluyveromyces lactis	?	-	?

Synonyms

Synonyms	Comment	Organism
Kex-1 endopeptidase	-	Kluyveromyces lactis
Kex-1 protease	-	Kluyveromyces lactis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	cleavage assay	Kluyveromyces lactis

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Release 2023.1 (January 2023)