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Literature summary for 3.4.16.2 extracted from

  • Abeywickrema, P.D.; Patel, S.B.; Byrne, N.J.; Diehl, R.E.; Hall, D.L.; Ford, R.E.; Rickert, K.W.; Reid, J.C.; Shipman, J.M.; Geissler, W.M.; Pryor, K.D.; SinhaRoy, R.; Soisson, S.M.; Lumb, K.J.; Sharma, S.
    Expression, purification and crystallization of human prolylcarboxypeptidase (2010), Acta Crystallogr. Sect. F, 66, 702-705.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.16.2

Cloned(Commentary)

Cloned (Comment) Organism
PrCP is cloned with an N-terminal FLAG tag and a C-terminal heptahistidine tag under the trypsin II signal sequence in pcDNA5/FRT and expressed in stably transfected CHO cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallization of glycosylated human PrCP from stably transformed CHO cells is described. Purified PrCP yields crystals belonging to space group R32, with unit-cell parameters a = b = 181.14, c = 240.13 A, that diffract to better than 2.8 A resolution Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
 Homo sapiens 5764
-

Organism

Organism UniProt Comment Textmining
Homo sapiens P42785
-
-

Purification (Commentary)

Purification (Comment) Organism
using Ni-affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7-methoxycoumarin-4-acetyl-Pro-Pro-Lys + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
PRCP
-
 Homo sapiens
prolylcarboxypeptidase
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
additional information
-
 assay at room temperature Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 assay at Homo sapiens