Inhibitors | Comment | Organism | Structure |
---|---|---|---|
angiotensin II | - |
Homo sapiens | |
Arg-Pro-Pro | inhibition of enzyme form I and II, enzyme form III is not inhibited | Homo sapiens | |
bradykinin | - |
Homo sapiens | |
bradykinin potentiator C | inhibition of enzyme form I and II, enzyme form III is not inhibited | Homo sapiens | |
EDTA | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
hippuryl-His-Leu | 100 mM borate/sodium carbonate buffer, pH 7.8 | Homo sapiens | |
1.1 | - |
hippuryl-His-Leu | 10 mM potassium phosphate buffer, pH 8.3, enzyme form I, II, and III | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
98000 | - |
enzyme form III, gel filtration | Homo sapiens |
180000 | - |
enzyme form II, gel filtration | Homo sapiens |
290000 | - |
enzyme form I, gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lung | 3 different enzyme forms: I, II, and III | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
angiotensin I + H2O | - |
Homo sapiens | angiotensin II + His-Leu | - |
? | |
bradykinin + H2O | - |
Homo sapiens | Phe-Arg + Ser-Pro + Arg-Pro-Pro-Gly-Phe | - |
? | |
hippuryl-His-Leu + H2O | - |
Homo sapiens | hippuric acid + His-Leu | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
10 min, enzyme form I and II lose 90% of the hydrolytic activity with hippuryl-His-Leu as substrate, enzyme form III loses 45% of its activity | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | 8 | hydrolysis of hippuryl-His-Leu, borate/sodium carbonate buffer | Homo sapiens |