Literature summary for 3.4.14.5 extracted from

Roppongi, S.; Suzuki, Y.; Tateoka, C.; Fujimoto, M.; Morisawa, S.; Iizuka, I.; Nakamura, A.; Honma, N.; Shida, Y.; Ogasawara, W.; Tanaka, N.; Sakamoto, Y.; Nonaka, T.
Crystal structures of a bacterial dipeptidyl peptidase IV reveal a novel substrate recognition mechanism distinct from that of mammalian orthologues (2018), Sci. Rep., 8, 2714 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Pseudoxanthomonas mexicana

Crystallization (Commentary)

Crystallization (Comment)	Organism
structures of free form and in complexes with dipeptides Lys-Pro or Ile-Pro as well as with a non-peptidyl inhibitor at 1.90 to 2.47 A resolution. Acyl-enzyme intermediates are observed for the dipeptide complexes, whereas tetrahedral intermediates are reported for the oligopeptide complexes of mammalian DPP IVs	Pseudoxanthomonas mexicana
to 1.9 A resolution, in complex with peptide in complex with the tripeptide Lys-Pro-Tyr	Pseudoxanthomonas mexicana

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0194	-	Gly-L-Pro-7-amido-4-methylcoumarin	pH 8.0, 25°C	Pseudoxanthomonas mexicana

Organism

Organism	UniProt	Comment	Textmining
Pseudoxanthomonas mexicana	Q6F3I7	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Gly-L-Pro-7-amido-4-methylcoumarin + H2O	-	Pseudoxanthomonas mexicana	Gly-L-Pro + 7-amino-4-methylcoumarin	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 82000, calculated from sequence and crystallization data	Pseudoxanthomonas mexicana

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
19.2	-	Gly-L-Pro-7-amido-4-methylcoumarin	pH 8.0, 25°C	Pseudoxanthomonas mexicana

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
989	-	Gly-L-Pro-7-amido-4-methylcoumarin	pH 8.0, 25°C	Pseudoxanthomonas mexicana

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Release 2023.1 (January 2023)