Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.14.5 extracted from

  • Tang, H.K.; Tang, H.Y.; Hsu, S.C.; Chu, Y.R.; Chien, C.H.; Shu, C.H.; Chen, X.
    Biochemical properties and expression profile of human prolyl dipeptidase DPP9 (2009), Arch. Biochem. Biophys., 485, 120-127.
    View publication on PubMed
Search for more literature for 3.4.14.5

Cloned(Commentary)

Cloned (Comment) Organism
DPP9 is expressed in baculovirus-infected Sf9 insect cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
F822A the DPP8 mutation abolishes the enzymatic activity without disrupting its quaternary structure Homo sapiens
F842A the DPP9 mutation abolishes the enzymatic activity without disrupting its quaternary structure Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information when assayed against L-Ala-L-Pro-p-nitroanilide or Gly-L-Pro-p-nitroanilide, the activity of DPP9 is not affected by the presence of reducing agents, such as 1 mM dithiothreitol or 18 mM beta-mercaptoethanol Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.008
-
 L-Lys-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens
0.014
-
 L-Leu-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens
0.014
-
 L-Arg-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens
0.018
-
 L-Met-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens
0.073
-
 L-Lys-L-Pro-p-nitroanilide mutant enzyme F842A, in PBS, pH 8.0 Homo sapiens
0.099
-
 L-Ala-L-Pro-p-nitroanilide wild type , in PBS, pH 8.0 Homo sapiens
0.102
-
 Gly-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens
0.127
-
 L-Leu-L-Pro-p-nitroanilide mutant enzyme F842A, in PBS, pH 8.0 Homo sapiens
0.189
-
 L-Ser-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens
0.337
-
 L-Asn-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens
0.432
-
 L-Ala-L-Pro-p-nitroanilide mutant enzyme F842A, in PBS, pH 8.0 Homo sapiens
1.244
-
 L-Glu-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Homo sapiens 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
95000
-
 2 * 95000, SDS-PAGE Homo sapiens
189000
-
 gel filtration Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Strep-Tactin resin purification system Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
blood
-
 Homo sapiens
-
epithelial cell
-
 Homo sapiens
-
fibroblast
-
 Homo sapiens
-
PBMC cell line
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.01
-
 crude extract, in PBS, pH 8.0 Homo sapiens
0.8
-
 after 80fold purification, in PBS, pH 8.0 Homo sapiens

Storage Stability

Storage Stability Organism
-80°C, purified enzyme, 1 month, no loss of activity Homo sapiens
22°C, purified enzyme, 12 h, no loss of activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-L-Pro-p-nitroanilide + H2O
-
 Homo sapiens L-Lys-L-Pro + p-nitroaniline
-
 ?
L-Ala-L-Pro-p-nitroanilide + H2O
-
 Homo sapiens L-Ala-L-Pro + p-nitroaniline
-
 ?
L-Arg-L-Pro-p-nitroanilide + H2O
-
 Homo sapiens L-Arg-L-Pro + p-nitroaniline
-
 ?
L-Asn-L-Pro-p-nitroanilide + H2O
-
 Homo sapiens L-Asn-L-Pro + p-nitroaniline
-
 ?
L-Asp-L-Pro-p-nitroanilide + H2O least preferred substrate Homo sapiens L-Asp-L-Pro + p-nitroaniline
-
 ?
L-Glu-L-Pro-p-nitroanilide + H2O
-
 Homo sapiens L-Glu-L-Pro + p-nitroaniline
-
 ?
L-Leu-L-Pro-p-nitroanilide + H2O
-
 Homo sapiens L-Leu-L-Pro + p-nitroaniline
-
 ?
L-Lys-L-Pro-p-nitroanilide + H2O
-
 Homo sapiens L-Lys-L-Pro + p-nitroaniline
-
 ?
L-Lys-L-Pro-p-nitroanilide + H2O most preferred substrate Homo sapiens L-Lys-L-Pro + p-nitroaniline
-
 ?
L-Met-L-Pro-p-nitroanilide + H2O
-
 Homo sapiens L-Met-L-Pro + p-nitroaniline
-
 ?
L-Ser-L-Pro-p-nitroanilide + H2O
-
 Homo sapiens L-Ser-L-Pro + p-nitroaniline
-
 ?
additional information no endopeptidase or tripeptidase activity is detected for DPP9, tested against Z-Gly-L-Pro-p-nitroanilide, L-Ala-L-Ala-L-Ala-p-nitroanilide, and L-Ala-L-Ala-L-Pro-p-nitroanilide Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 95000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
dipeptidyl peptidase 8
-
 Homo sapiens
dipeptidyl peptidase 9
-
 Homo sapiens
DPP8 DPP8 is a prolyl dipeptidases, cleaving the peptide bond after the penultimate proline residue Homo sapiens
DPP9 DPP9 is a prolyl dipeptidases, cleaving the peptide bond after the penultimate proline residue Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
7.5 8 the activity decreases above pH 8.0 or below pH 7.5 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.4
-
 L-Lys-L-Pro-p-nitroanilide mutant enzyme F842A, in PBS, pH 8.0 Homo sapiens
0.6
-
 L-Ala-L-Pro-p-nitroanilide mutant enzyme F842A, in PBS, pH 8.0 Homo sapiens
1.9
-
 L-Leu-L-Pro-p-nitroanilide mutant enzyme F842A, in PBS, pH 8.0 Homo sapiens
5
-
 L-Lys-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens
6.3
-
 L-Leu-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens
7
-
 L-Glu-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens
7.7
-
 L-Arg-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens
8.7
-
 L-Met-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens
11
-
 L-Asn-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens
18
-
 L-Ser-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens
20.3
-
 Gly-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens
33
-
 L-Ala-L-Pro-p-nitroanilide wild type enzyme, in PBS, pH 8.0 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
 Homo sapiens