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Literature summary for 3.4.14.5 extracted from

  • Fujimura, S.; Shibata, Y.; Hirai, K.; Ueda, O.
    Dipeptidyl peptidase IV of Streptococcus anginosus: purification and characterization (2005), Eur. J. Med. Res., 10, 278-282.
    View publication on PubMed
Search for more literature for 3.4.14.5

Activating Compound

Activating Compound Comment Organism Structure
bestatin 0.2 mM, 10% activation Streptococcus anginosus
EDTA 1.0 mM, 8% activation Streptococcus anginosus
leupeptin 0.2 mM, 8% activation Streptococcus anginosus

Inhibitors

Inhibitors Comment Organism Structure
3,4-dichloroisocoumarine 1.0 mM, 97% inhibition Streptococcus anginosus
antipain 0.2 mM, 17% inhibition Streptococcus anginosus
Cu2+ 1.0 mM, 15% inhibition Streptococcus anginosus
diisopropylfluorophosphate 1.0 mM, complete inhibition Streptococcus anginosus
Mn2+ 1.0 mM, 22% inhibition Streptococcus anginosus
SDS 1.0% complete inhibition Streptococcus anginosus
Triton X-100 1.0%, 32% inhibition Streptococcus anginosus
Zn2+ 1.0 mM, 22% inhibition Streptococcus anginosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.179
-
 Met-Pro-4-nitroanilide pH 7.5, 37°C Streptococcus anginosus
0.217
-
 His-Pro-4-nitroanilide pH 7.5, 37°C Streptococcus anginosus
0.217
-
 Ser-Pro-4-nitroanilide pH 7.5, 37°C Streptococcus anginosus
0.33
-
 Phe-Pro-4-nitroanilide pH 7.5, 37°C Streptococcus anginosus
0.37
-
 Lys-Pro-4-nitroanilide pH 7.5, 37°C Streptococcus anginosus
0.556
-
 Gly-Pro-4-nitroanilide pH 7.5, 37°C Streptococcus anginosus
0.714
-
 Glu-Pro-4-nitroanilide pH 7.5, 37°C Streptococcus anginosus
0.75
-
 Ala-Pro-4-nitroanilide pH 7.5, 37°C Streptococcus anginosus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 1.0 mM, 7% activation Streptococcus anginosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
84000
-
 x * 84000, SDS-PAGE Streptococcus anginosus

Organism

Organism UniProt Comment Textmining
Streptococcus anginosus
-
 NCTC 10713
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptococcus anginosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.75
-
-
 Streptococcus anginosus

Storage Stability

Storage Stability Organism
-40°C, stable for at least 1 month Streptococcus anginosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Pro-4-nitroanilide + H2O
-
 Streptococcus anginosus Ala-Pro + 4-nitroaniline
-
 ?
Glu-Pro-4-nitroanilide + H2O
-
 Streptococcus anginosus Glu-Pro + 4-nitroaniline
-
 ?
Gly-Pro-4-nitroanilide + H2O
-
 Streptococcus anginosus Gly-Pro + 4-nitroaniline
-
 ?
His-Pro-4-nitroanilide + H2O
-
 Streptococcus anginosus His-Pro + 4-nitroaniline
-
 ?
Lys-Pro-4-nitroanilide + H2O
-
 Streptococcus anginosus Lys-Pro + 4-nitroaniline
-
 ?
Met-Pro-4-nitroanilide + H2O
-
 Streptococcus anginosus Met-Pro + 4-nitroaniline
-
 ?
additional information no hydrolysis of Ala-Ala-Pro-4-nitroanilide, Ala-Phe-Pro-4-nitroanilide, Pro-4-nitroanilide, Leu-4-nitroanilide, Arg, 4-nitroanilide, Ala-Ala-Ala-4-nitroanilide Streptococcus anginosus ?
-
 ?
Phe-Pro-4-nitroanilide + H2O
-
 Streptococcus anginosus Phe-Pro + 4-nitroaniline
-
 ?
Ser-Pro-4-nitroanilide + H2O
-
 Streptococcus anginosus Ser-Pro + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
? x * 84000, SDS-PAGE Streptococcus anginosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 10 h, stable Streptococcus anginosus
50
-
 15 min, 94% loss of activity Streptococcus anginosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Streptococcus anginosus

pI Value

Organism Comment pI Value Maximum pI Value
Streptococcus anginosus isoelectric focusing, pH-range 4.0-6.0
-
 4.9