Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutants in CHO cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
C299G | site-directed mutagenesis, similar to the wild-type enzyme | Rattus norvegicus |
C326G | site-directed mutagenesis, total inhibition of surface expression, catalytically inactive, half-life of the mutant enzyme is reduced by over 90% | Rattus norvegicus |
C337S | site-directed mutagenesis, weak expression, reduced activity, half-life of the mutant enzyme is reduced by over 90% | Rattus norvegicus |
C383G | site-directed mutagenesis, similar to the wild-type enzyme | Rattus norvegicus |
C395S | site-directed mutagenesis, similar to the wild-type enzyme | Rattus norvegicus |
C445S | site-directed mutagenesis, total inhibition of surface expression, catalytically inactive, half-life of the mutant enzyme is reduced by over 90% | Rattus norvegicus |
C448S | site-directed mutagenesis, total inhibition of surface expression, catalytically inactive, half-life of the mutant enzyme is reduced by over 90% | Rattus norvegicus |
C455G | site-directed mutagenesis, weak expression, reduced activity, half-life of the mutant enzyme is reduced by over 90% | Rattus norvegicus |
C473S | site-directed mutagenesis, weak expression, reduced activity, half-life of the mutant enzyme is reduced by over 90% | Rattus norvegicus |
C552S | site-directed mutagenesis, weak expression, reduced activity, half-life of the mutant enzyme is reduced by over 90% | Rattus norvegicus |
C650G | site-directed mutagenesis, similar to the wild-type enzyme | Rattus norvegicus |
C763S | site-directed mutagenesis, similar to the wild-type enzyme | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | processing kinetics of wild-type and mutants | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
endoplasmic reticulum | - |
Rattus norvegicus | 5783 | - |
Golgi apparatus | - |
Rattus norvegicus | 5794 | - |
membrane | type II transmembrane glycoprotein, cell surface | Rattus norvegicus | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
2 * 110000, wild-type enzyme | Rattus norvegicus |
210000 | - |
wild-type enzyme | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
no activity in Cricetulus griseus | - |
chinese hamster, ovary cells | - |
Rattus norvegicus | P14740 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | type II transmembrane glycoprotein | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
lymphocyte | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gly-Pro-4-nitroanilide + H2O | - |
Rattus norvegicus | Gly-Pro + 4-nitroaniline | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 110000, wild-type enzyme | Rattus norvegicus |
More | domain structure involving cysteine residues and disulfide bridges, structure-function relationship, overview, cysteine residues 326, 337,445, 448, 455, 473, and 552 are essential for the correct folding and intracellular trafficking of the enzyme, structure of wild-type compared to the mutant enzymes | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
CD26 | dipeptidyl-peptidase IV is identical with the adenosine deaminase binding protein CD26 on lymphocytes | Rattus norvegicus |
dipeptidyl peptidase IV | - |
Rattus norvegicus |
DPPIV | - |
Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Rattus norvegicus |