Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.4.14.5 extracted from

  • Dobers, J.; Grams, S.; Reutter, W.; Fan, H.
    Roles of cysteines in rat dipeptidyl peptidase IV/CD26 in processing and proteolytic activity (2000), Eur. J. Biochem., 267, 5093-5100.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutants in CHO cells Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
C299G site-directed mutagenesis, similar to the wild-type enzyme Rattus norvegicus
C326G site-directed mutagenesis, total inhibition of surface expression, catalytically inactive, half-life of the mutant enzyme is reduced by over 90% Rattus norvegicus
C337S site-directed mutagenesis, weak expression, reduced activity, half-life of the mutant enzyme is reduced by over 90% Rattus norvegicus
C383G site-directed mutagenesis, similar to the wild-type enzyme Rattus norvegicus
C395S site-directed mutagenesis, similar to the wild-type enzyme Rattus norvegicus
C445S site-directed mutagenesis, total inhibition of surface expression, catalytically inactive, half-life of the mutant enzyme is reduced by over 90% Rattus norvegicus
C448S site-directed mutagenesis, total inhibition of surface expression, catalytically inactive, half-life of the mutant enzyme is reduced by over 90% Rattus norvegicus
C455G site-directed mutagenesis, weak expression, reduced activity, half-life of the mutant enzyme is reduced by over 90% Rattus norvegicus
C473S site-directed mutagenesis, weak expression, reduced activity, half-life of the mutant enzyme is reduced by over 90% Rattus norvegicus
C552S site-directed mutagenesis, weak expression, reduced activity, half-life of the mutant enzyme is reduced by over 90% Rattus norvegicus
C650G site-directed mutagenesis, similar to the wild-type enzyme Rattus norvegicus
C763S site-directed mutagenesis, similar to the wild-type enzyme Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information processing kinetics of wild-type and mutants Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum
-
Rattus norvegicus 5783
-
Golgi apparatus
-
Rattus norvegicus 5794
-
membrane type II transmembrane glycoprotein, cell surface Rattus norvegicus 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
110000
-
2 * 110000, wild-type enzyme Rattus norvegicus
210000
-
wild-type enzyme Rattus norvegicus

Organism

Organism UniProt Comment Textmining
no activity in Cricetulus griseus
-
chinese hamster, ovary cells
-
Rattus norvegicus P14740
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein type II transmembrane glycoprotein Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-
lymphocyte
-
Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-Pro-4-nitroanilide + H2O
-
Rattus norvegicus Gly-Pro + 4-nitroaniline
-
?

Subunits

Subunits Comment Organism
dimer 2 * 110000, wild-type enzyme Rattus norvegicus
More domain structure involving cysteine residues and disulfide bridges, structure-function relationship, overview, cysteine residues 326, 337,445, 448, 455, 473, and 552 are essential for the correct folding and intracellular trafficking of the enzyme, structure of wild-type compared to the mutant enzymes Rattus norvegicus

Synonyms

Synonyms Comment Organism
CD26 dipeptidyl-peptidase IV is identical with the adenosine deaminase binding protein CD26 on lymphocytes Rattus norvegicus
dipeptidyl peptidase IV
-
Rattus norvegicus
DPPIV
-
Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Rattus norvegicus