Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Diprotin A | - |
Rattus norvegicus | |
hemorphin-4 peptide | 12% inhibition at 0.03 mM, endogenous blood-derived peptide belonging to the family of atypical opioidic peptides released from sequentially hydrolyzed hemoglobin, amino acid sequence YPWT, competitive | Rattus norvegicus | |
hemorphin-5 peptide | 18% inhibition at 0.03 mM, endogenous blood-derived peptide belonging to the family of atypical opioidic peptides released from sequentially hydrolyzed hemoglobin, amino acid sequence YPWTQ, competitive | Rattus norvegicus | |
hemorphin-7 peptide | 30% inhibition at 0.03 mM, endogenous blood-derived peptide belonging to the family of atypical opioidic peptides released from sequentially hydrolyzed hemoglobin, amino acid sequence YPWTQRF, selective and competitive | Rattus norvegicus | |
LVV-hemorphin-7 peptide | 9% inhibition at 0.03 mM, endogenous blood-derived peptide belonging to the family of atypical opioidic peptides released from sequentially hydrolyzed hemoglobin, amino acid sequence LVVYPWTQRF, the additional leucine residue reduces the inhibitory potential | Rattus norvegicus | |
additional information | inhibitory mechanism, the N-terminus sequence of hemorphins influence the interaction between hemorphins and the enzyme | Rattus norvegicus | |
VV-hemorphin-5 peptide | 10% inhibition at 0.03 mM, endogenous blood-derived peptide belonging to the family of atypical opioidic peptides released from sequentially hydrolyzed hemoglobin, amino acid sequence VVYPWTQ | Rattus norvegicus | |
VV-hemorphin-6 peptide | 15% inhibition at 0.03 mM, endogenous blood-derived peptide belonging to the family of atypical opioidic peptides released from sequentially hydrolyzed hemoglobin, amino acid sequence VVYPWTQR | Rattus norvegicus | |
VV-hemorphin-7 peptide | 27% inhibition at 0.03 mM, endogenous blood-derived peptide belonging to the family of atypical opioidic peptides released from sequentially hydrolyzed hemoglobin, amino acid sequence VVYPWTQRF | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0387 | - |
hemorphin-7 | pH 8.0, 37°C, microsomes | Rattus norvegicus | |
0.38 | - |
Gly-Pro-4-nitroanilide | pH 8.0, 37°C, microsomes | Rattus norvegicus | |
6.4 | - |
hemorphin-5 | pH 8.0, 37°C, microsomes | Rattus norvegicus | |
13.5 | - |
hemorphin-4 | pH 8.0, 37°C, microsomes | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Rattus norvegicus | 16020 | - |
microsome | - |
Rattus norvegicus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | hemorphins may represent endogenous regulators of the enzyme activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
male Wistar rats | - |
Purification (Comment) | Organism |
---|---|
partial, preparation of microsomal fraction | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Gly-Pro-4-nitroanilide + H2O | - |
Rattus norvegicus | Gly-Pro + 4-nitroaniline | - |
? | |
hemorphin-4 + H2O | - |
Rattus norvegicus | YP + WT | - |
? | |
hemorphin-5 + H2O | - |
Rattus norvegicus | YP + WTQ | - |
? | |
hemorphin-7 + H2O | - |
Rattus norvegicus | YP + WTQRF | - |
? | |
additional information | LVV-hemorphin-7 and LVV-hemorphin-6 are no substrates for the enzyme | Rattus norvegicus | ? | - |
? | |
additional information | hemorphins may represent endogenous regulators of the enzyme activity | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
dipeptidyl peptidase IV | - |
Rattus norvegicus |
DPPIV | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.3 | - |
hemorphin-7 | pH 8.0, 37°C, microsomes | Rattus norvegicus | |
52.3 | - |
Gly-Pro-4-nitroanilide | pH 8.0, 37°C, microsomes | Rattus norvegicus | |
1770 | - |
hemorphin-4 | pH 8.0, 37°C, microsomes | Rattus norvegicus | |
1774 | - |
hemorphin-4 | pH 8.0, 37°C, microsomes | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibition kinetics | Rattus norvegicus | |
0.029 | - |
VV-hemorphin-7 peptide | pH 8.0, 37°C, microsomes | Rattus norvegicus | |
0.033 | - |
hemorphin-7 peptide | pH 8.0, 37°C, microsomes | Rattus norvegicus |