General Stability | Organism |
---|---|
complete loss of activity when frozen in imidazole buffer | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ala-Pro | - |
Rattus norvegicus | |
Cd2+ | - |
Rattus norvegicus | |
Hg2+ | - |
Rattus norvegicus | |
L-His-L-Ala | - |
Rattus norvegicus | |
L-Leu-L-Ala | - |
Rattus norvegicus | |
L-Leu-L-Leu | - |
Rattus norvegicus | |
L-Leu-L-Pro | - |
Rattus norvegicus | |
L-Lys-L-Ala | - |
Rattus norvegicus | |
L-Met-L-Ala | - |
Rattus norvegicus | |
L-Met-L-Pro | - |
Rattus norvegicus | |
L-Phe-L-Ala | - |
Rattus norvegicus | |
L-Phe-L-Pro | - |
Rattus norvegicus | |
L-Val-L-Ala | - |
Rattus norvegicus | |
L-Val-L-Pro | - |
Rattus norvegicus | |
L-Val-L-Val | - |
Rattus norvegicus | |
Pro-Ala | hydrolysis of L-Pro-L-Ala | Rattus norvegicus | |
Zn2+ | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
L-Leu-L-Pro-Gly-Gly | - |
Rattus norvegicus | |
0.17 | - |
L-Ala-L-Pro-Gly | - |
Rattus norvegicus | |
0.2 | - |
L-Lys-L-Ala-2-naphthylamide | - |
Rattus norvegicus | |
0.24 | - |
L-Leu-L-Ala-2-naphthylamide | - |
Rattus norvegicus | |
0.26 | - |
Gly-L-Pro-2-naphthylamide | - |
Rattus norvegicus | |
0.28 | - |
Gly-L-Pro-Gly-Gly | - |
Rattus norvegicus | |
0.29 | - |
Hly-Pro-2-naphthylamide | - |
Rattus norvegicus | |
0.31 | - |
Gly-L-Pro-L-Ala | - |
Rattus norvegicus | |
0.35 | - |
L-Leu-L-Leu-L-Leu | - |
Rattus norvegicus | |
0.36 | - |
L-Phe-L-Pro-2-naphthylamide | - |
Rattus norvegicus | |
1.25 | - |
L-Leu-L-Leu-Gly | - |
Rattus norvegicus | |
2.5 | - |
L-Ala-L-Ala-L-Ala | - |
Rattus norvegicus | |
5 | - |
Gly-L-Ala-2-naphthylamide | - |
Rattus norvegicus | |
7.5 | - |
L-Ala-L-Ala-2-naphthylamide | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
brush border | - |
Rattus norvegicus | 5903 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
intestine | - |
Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
-20°C, 50 mM ammonium acetate buffer, pH 7, stable for several months | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Ala-2-naphthylamide + H2O | - |
Rattus norvegicus | Ala-Ala + 2-naphthylamine | - |
? | |
Ala-Ala-Ala + H2O | L-Ala-L-Ala-L-Ala | Rattus norvegicus | Ala-Ala + Ala | - |
? | |
Ala-Pro-Gly + H2O | - |
Rattus norvegicus | Ala-Pro + Gly | - |
? | |
Gly-L-Ala-2-naphthylamide + H2O | - |
Rattus norvegicus | Gly-L-Ala + 2-naphthylamine | - |
? | |
Gly-L-Pro-Gly-Gly + H2O | - |
Rattus norvegicus | ? | - |
? | |
Gly-L-Pro-L-Ala + H2O | - |
Rattus norvegicus | Gly-L-Pro + L-Ala | - |
? | |
Gly-Pro-2-naphthylamide + H2O | L-Gly-Pro-2-naphthylamide | Rattus norvegicus | Gly-Pro + 2-naphthylamine | - |
? | |
Hly-Pro-2-naphthylamide | - |
Rattus norvegicus | ? | - |
? | |
L-Leu-L-Ala-2-naphthylamide + H2O | - |
Rattus norvegicus | L-Leu-L-Ala + 2-naphthylamine | - |
? | |
L-Leu-L-Leu-Gly + H2O | - |
Rattus norvegicus | ? | - |
? | |
L-Leu-L-Leu-L-Leu + H2O | - |
Rattus norvegicus | ? | - |
? | |
L-Leu-L-Pro-Gly-Gly + H2O | - |
Rattus norvegicus | ? | - |
? | |
L-Lys-L-Ala-2-naphthylamide + H2O | - |
Rattus norvegicus | L-Lys-L-Ala + 2-naphthylamine | - |
? | |
additional information | peptides with proline penultimate to the amino terminus are more favored substrates while those substrates having Leu at both the penultimate position and amino terminus Leu-Leu | Rattus norvegicus | ? | - |
? | |
Phe-Pro-2-naphthylamide + H2O | - |
Rattus norvegicus | Phe-Pro + 2-naphthylamine | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
stable up to | Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
L-Leu-L-Leu-Gly | - |
Rattus norvegicus | |
2.7 | - |
L-Leu-L-Leu-L-Leu | - |
Rattus norvegicus | |
5.6 | - |
Gly-L-Ala-2-naphthylamide | - |
Rattus norvegicus | |
6.3 | - |
L-Lys-L-Ala-2-naphthylamide | - |
Rattus norvegicus | |
11.2 | - |
L-Leu-L-Ala-2-naphthylamide | - |
Rattus norvegicus | |
15.1 | - |
L-Ala-L-Ala-L-Ala | - |
Rattus norvegicus | |
31.3 | - |
Gly-L-Pro-Gly-Gly | - |
Rattus norvegicus | |
46.2 | - |
L-Ala-L-Pro-Gly | - |
Rattus norvegicus | |
52.3 | - |
L-Ala-L-Ala-2-naphthylamide | - |
Rattus norvegicus | |
82.3 | - |
Gly-L-Pro-L-Ala | - |
Rattus norvegicus | |
82.8 | - |
L-Leu-L-Pro-Gly-Gly | - |
Rattus norvegicus | |
128 | - |
Gly-L-Pro-2-naphthylamide | - |
Rattus norvegicus | |
990 | - |
L-Phe-L-Pro-2-naphthylamide | - |
Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | 8.6 | ,hydrolysis of Gly-Pro-2-naphthylamide | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.5 | pH 6.5: about 60% of maximal activity, pH 9.5: about 45% of maximal activity | Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
rapid inactivation below | Rattus norvegicus |
11 | - |
stable up to | Rattus norvegicus |