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Literature summary for 3.4.14.5 extracted from

  • Bella, A.M.; Erickson, R.H.; Kim, Y.S.
    Rat intestinal brush border membrane dipeptidyl-aminopeptidase IV: kinetic properties and substrate specificities of the purified enzyme (1982), Arch. Biochem. Biophys., 218, 156-162.
    View publication on PubMed

General Stability

General Stability Organism
complete loss of activity when frozen in imidazole buffer Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
Ala-Pro
-
Rattus norvegicus
Cd2+
-
Rattus norvegicus
Hg2+
-
Rattus norvegicus
L-His-L-Ala
-
Rattus norvegicus
L-Leu-L-Ala
-
Rattus norvegicus
L-Leu-L-Leu
-
Rattus norvegicus
L-Leu-L-Pro
-
Rattus norvegicus
L-Lys-L-Ala
-
Rattus norvegicus
L-Met-L-Ala
-
Rattus norvegicus
L-Met-L-Pro
-
Rattus norvegicus
L-Phe-L-Ala
-
Rattus norvegicus
L-Phe-L-Pro
-
Rattus norvegicus
L-Val-L-Ala
-
Rattus norvegicus
L-Val-L-Pro
-
Rattus norvegicus
L-Val-L-Val
-
Rattus norvegicus
Pro-Ala hydrolysis of L-Pro-L-Ala Rattus norvegicus
Zn2+
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
L-Leu-L-Pro-Gly-Gly
-
Rattus norvegicus
0.17
-
L-Ala-L-Pro-Gly
-
Rattus norvegicus
0.2
-
L-Lys-L-Ala-2-naphthylamide
-
Rattus norvegicus
0.24
-
L-Leu-L-Ala-2-naphthylamide
-
Rattus norvegicus
0.26
-
Gly-L-Pro-2-naphthylamide
-
Rattus norvegicus
0.28
-
Gly-L-Pro-Gly-Gly
-
Rattus norvegicus
0.29
-
Hly-Pro-2-naphthylamide
-
Rattus norvegicus
0.31
-
Gly-L-Pro-L-Ala
-
Rattus norvegicus
0.35
-
L-Leu-L-Leu-L-Leu
-
Rattus norvegicus
0.36
-
L-Phe-L-Pro-2-naphthylamide
-
Rattus norvegicus
1.25
-
L-Leu-L-Leu-Gly
-
Rattus norvegicus
2.5
-
L-Ala-L-Ala-L-Ala
-
Rattus norvegicus
5
-
Gly-L-Ala-2-naphthylamide
-
Rattus norvegicus
7.5
-
L-Ala-L-Ala-2-naphthylamide
-
Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
brush border
-
Rattus norvegicus 5903
-

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
intestine
-
Rattus norvegicus
-

Storage Stability

Storage Stability Organism
-20°C, 50 mM ammonium acetate buffer, pH 7, stable for several months Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-2-naphthylamide + H2O
-
Rattus norvegicus Ala-Ala + 2-naphthylamine
-
?
Ala-Ala-Ala + H2O L-Ala-L-Ala-L-Ala Rattus norvegicus Ala-Ala + Ala
-
?
Ala-Pro-Gly + H2O
-
Rattus norvegicus Ala-Pro + Gly
-
?
Gly-L-Ala-2-naphthylamide + H2O
-
Rattus norvegicus Gly-L-Ala + 2-naphthylamine
-
?
Gly-L-Pro-Gly-Gly + H2O
-
Rattus norvegicus ?
-
?
Gly-L-Pro-L-Ala + H2O
-
Rattus norvegicus Gly-L-Pro + L-Ala
-
?
Gly-Pro-2-naphthylamide + H2O L-Gly-Pro-2-naphthylamide Rattus norvegicus Gly-Pro + 2-naphthylamine
-
?
Hly-Pro-2-naphthylamide
-
Rattus norvegicus ?
-
?
L-Leu-L-Ala-2-naphthylamide + H2O
-
Rattus norvegicus L-Leu-L-Ala + 2-naphthylamine
-
?
L-Leu-L-Leu-Gly + H2O
-
Rattus norvegicus ?
-
?
L-Leu-L-Leu-L-Leu + H2O
-
Rattus norvegicus ?
-
?
L-Leu-L-Pro-Gly-Gly + H2O
-
Rattus norvegicus ?
-
?
L-Lys-L-Ala-2-naphthylamide + H2O
-
Rattus norvegicus L-Lys-L-Ala + 2-naphthylamine
-
?
additional information peptides with proline penultimate to the amino terminus are more favored substrates while those substrates having Leu at both the penultimate position and amino terminus Leu-Leu Rattus norvegicus ?
-
?
Phe-Pro-2-naphthylamide + H2O
-
Rattus norvegicus Phe-Pro + 2-naphthylamine
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
stable up to Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5
-
L-Leu-L-Leu-Gly
-
Rattus norvegicus
2.7
-
L-Leu-L-Leu-L-Leu
-
Rattus norvegicus
5.6
-
Gly-L-Ala-2-naphthylamide
-
Rattus norvegicus
6.3
-
L-Lys-L-Ala-2-naphthylamide
-
Rattus norvegicus
11.2
-
L-Leu-L-Ala-2-naphthylamide
-
Rattus norvegicus
15.1
-
L-Ala-L-Ala-L-Ala
-
Rattus norvegicus
31.3
-
Gly-L-Pro-Gly-Gly
-
Rattus norvegicus
46.2
-
L-Ala-L-Pro-Gly
-
Rattus norvegicus
52.3
-
L-Ala-L-Ala-2-naphthylamide
-
Rattus norvegicus
82.3
-
Gly-L-Pro-L-Ala
-
Rattus norvegicus
82.8
-
L-Leu-L-Pro-Gly-Gly
-
Rattus norvegicus
128
-
Gly-L-Pro-2-naphthylamide
-
Rattus norvegicus
990
-
L-Phe-L-Pro-2-naphthylamide
-
Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2 8.6 ,hydrolysis of Gly-Pro-2-naphthylamide Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 9.5 pH 6.5: about 60% of maximal activity, pH 9.5: about 45% of maximal activity Rattus norvegicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
rapid inactivation below Rattus norvegicus
11
-
stable up to Rattus norvegicus