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Literature summary for 3.4.14.4 extracted from

  • Jajcanin-Jozic, N.; Deller, S.; Pavkov, T.; Macheroux, P.; Abramic, M.
    Identification of the reactive cysteine residues in yeast dipeptidyl peptidase III (2010), Biochimie, 92, 89-96.
    View publication on PubMed
Search for more literature for 3.4.14.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
C113S mutation of the cysteine residue Saccharomyces cerevisiae
C130S mutant, only existence of dimeric form Saccharomyces cerevisiae
C518S mutation of the cysteine residue Saccharomyces cerevisiae
C626S mutation of the cysteine residue Saccharomyces cerevisiae
C639S mutant, resistance against p-hydroxy-mercuribenzoate Saccharomyces cerevisiae
additional information DPPIII6His, construct with hexahistidine Saccharomyces cerevisiae

General Stability

General Stability Organism
4,4'-dithiodipyridine, inactivation of wild-type dipeptidyl peptidase III after incubation at pH 8.0 at 25°C, after 5 min incubation loss of 30% enzyme activity, after 15 min incubation loss of 62% enzyme activity Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
4,4'-dithiodipyridine inactivation of wild-type dipeptidyl peptidase III after incubation at pH 8.0 at 25°C, after 5 min incubation loss of 30% enzyme activity, after 15 min incubation loss of 62% enzyme activity Saccharomyces cerevisiae
Zn2+ 10 micromol, strong inhibition Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00889
-
 Arg-Arg-2-naphthylamide mutant C130S, pH 8.0, 37°C Saccharomyces cerevisiae
0.01041
-
 Arg-Arg-2-naphthylamide wild-type, pH 8.0, 37°C Saccharomyces cerevisiae
0.01097
-
 Arg-Arg-2-naphthylamide mutant C639S, pH 8.0, 37°C Saccharomyces cerevisiae
0.01191
-
 Arg-Arg-2-naphthylamide mutant C518S, pH 8.0, 37°C Saccharomyces cerevisiae
0.01518
-
 Arg-Arg-2-naphthylamide wild type, with His-tag, pH 8.0, 37°C Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 10-100 micromol, potent activation Saccharomyces cerevisiae
Zn2+ 10 micromol, strong inhibition Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82000
-
 SDS-PAGE Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae Q08225
-
-

Purification (Commentary)

Purification (Comment) Organism
gel filtration and FPLC on Mono Q column Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-2-naphthylamide + H2O
-
 Saccharomyces cerevisiae Ala-Ala + 2-naphthylamine
-
 ?
Ala-Phe-2-naphthylamide + H2O
-
 Saccharomyces cerevisiae Ala-Phe + 2-naphthylamine
-
 ?
Arg-Arg-2-naphthylamide + H2O assay at pH 8.0, 37°C Saccharomyces cerevisiae Arg-Arg + 2-naphthylamine
-
 ?
His-Ser-2-naphthylamide + H2O
-
 Saccharomyces cerevisiae His-Ser + 2-naphthylamine
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Saccharomyces cerevisiae
monomer
-
 Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
dipeptidyl peptidase III
-
 Saccharomyces cerevisiae
DPP III
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.09
-
 Arg-Arg-2-naphthylamide mutant C130S, pH 8.0, 37°C Saccharomyces cerevisiae
0.131
-
 Arg-Arg-2-naphthylamide mutant C518S, pH 8.0, 37°C Saccharomyces cerevisiae
0.249
-
 Arg-Arg-2-naphthylamide wild-type, with His-tag, pH 8.0, 37°C Saccharomyces cerevisiae
0.256
-
 Arg-Arg-2-naphthylamide mutant C639S, pH 8.0, 37°C Saccharomyces cerevisiae
0.334
-
 Arg-Arg-2-naphthylamide wild-type, pH 8.0, 37°C Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Saccharomyces cerevisiae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
10.21
-
 Arg-Arg-2-naphthylamide mutant C130S, pH 8.0, 37°C Saccharomyces cerevisiae
10.86
-
 Arg-Arg-2-naphthylamide mutant C518S, pH 8.0, 37°C Saccharomyces cerevisiae
16.74
-
 Arg-Arg-2-naphthylamide wild type, with His-tag, pH 8.0, 37°C Saccharomyces cerevisiae
23.88
-
 Arg-Arg-2-naphthylamide mutant C639S, pH 8.0, 37°C Saccharomyces cerevisiae
32.58
-
 Arg-Arg-2-naphthylamide wild-type, pH 8.0, 37°C Saccharomyces cerevisiae