Literature summary for 3.4.14.4 extracted from

Barsun, M.; Jajcanin, N.; Vukeli?, B.; Spoljari?, J.; Abrami?, M.
Human dipeptidyl peptidase III acts as a post-proline-cleaving enzyme on endomorphins (2007), Biol. Chem., 388, 343-348.

Search for more literature for 3.4.14.4

Inhibitors

Inhibitors	Comment	Organism	Structure
Gly-Gly-Phe-Leu	enkephalin fragment	Homo sapiens
Leu-Val-Val-Tyr-Pro-Trp-Thr-Gln-Arg	Leu-valorphin-Arg	Homo sapiens
Tyr(SO3H)-Gly-Gly-Phe-Leu	-	Homo sapiens
Tyr-Ala-Gly-Phe-Leu	Ala2-Leu-enkephalin	Homo sapiens
Tyr-D-Ala-Gly-Phe-Leu	D-Ala2-Leu-enkephalin	Homo sapiens
Tyr-Gly-Gly-Phe	enkephalin fragment	Homo sapiens
Tyr-Gly-Gly-Phe-Leu	Leu-enkephalin	Homo sapiens
Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile	dynorphin A 1-8	Homo sapiens
Tyr-Pro-Phe-Phe-NH2	endomorphin-2	Homo sapiens
Tyr-Pro-Phe-Val-Glu-Pro-Ile	human beta-casomorphin	Homo sapiens
Tyr-Pro-Trp-Phe-NH2	endomorphin-1	Homo sapiens
Val-Val-Tyr-Pro-Trp-Thr-Gln	valorphin	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0081	-	Tyr-Pro-Trp-Phe-NH2	endomorphin-1	Homo sapiens
0.0222	-	Arg-Arg-2-naphthylamide	-	Homo sapiens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	-	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
82000	-	determined by PAGE, native and denaturing conditions	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
the protein is purified from human erythrocytes	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
cerebrospinal fluid	-	Homo sapiens	-
erythrocyte	-	Homo sapiens	-
spinal cord	superficial laminae of the spinal cord dorsal horn	Homo sapiens	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
87.5	-	in the presence of 10 microM CoCl2	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Arg-Arg-2-naphthylamide + H2O	substrate used in the activity assay	Homo sapiens	Arg-Arg + 2-naphthylamine	-	?
Tyr-Pro-Trp-Phe-NH2 + H2O	endomorphin-1	Homo sapiens	Tyr-Pro + Trp-Phe-NH2	-	?

Subunits

Subunits	Comment	Organism
monomer	-	Homo sapiens

Synonyms

Synonyms	Comment	Organism
dipeptidyl arylamidase III	-	Homo sapiens
dipeptidyl peptidase III	-	Homo sapiens
DPP III	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	-	Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2	8	Arg-Arg-2-naphthylamide	-	Homo sapiens
5	-	Tyr-Pro-Trp-Phe-NH2	endomorphin-1	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	physiological condition, determination of Ki values and kinetic parameters of the hydrolysis of endomorphin-1	Homo sapiens
8.6	-	assay condition with the synthetic substrate Arg-Arg-2-naphthylamide, determination of specific activity	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.000049	-	Val-Val-Tyr-Pro-Trp-Thr-Gln	-	Homo sapiens
0.000375	-	Tyr-Ala-Gly-Phe-Leu	-	Homo sapiens
0.00056	-	Tyr-Pro-Phe-Val-Glu-Pro-Ile	-	Homo sapiens
0.00249	-	Tyr-Pro-Phe-Phe-NH2	-	Homo sapiens
0.00341	-	Leu-Val-Val-Tyr-Pro-Trp-Thr-Gln-Arg	-	Homo sapiens
0.00365	-	Tyr-Gly-Gly-Phe-Leu	-	Homo sapiens
0.004	-	Tyr-Gly-Gly-Phe	-	Homo sapiens
0.005	-	Tyr-Pro-Trp-Phe-NH2	-	Homo sapiens
0.0235	-	Gly-Gly-Phe-Leu	-	Homo sapiens
0.027	-	Tyr-D-Ala-Gly-Phe-Leu	-	Homo sapiens
0.0434	-	Tyr(SO3H)-Gly-Gly-Phe-Leu	-	Homo sapiens
0.0699	-	Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile	-	Homo sapiens

pI Value

Organism	Comment	pI Value Maximum	pI Value
Homo sapiens	-	-	4.5

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Release 2023.1 (January 2023)