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Literature summary for 3.4.14.4 extracted from

  • Abramic, M.; Schleuder, D.; Dolovcak, L.; Schroder, W.; Strupat, K.; Sagi, D.; Peter-Katalini, J.; Vitale, L.
    Human and rat dipeptidyl peptidase III: biochemical and mass spectrometric arguments for similarities and differences (2000), Biol. Chem., 381, 1233-1243.
    View publication on PubMed
Search for more literature for 3.4.14.4

Inhibitors

Inhibitors Comment Organism Structure
amastatin 30% inhibition at 0.1 mM Rattus norvegicus
angiotensin II
-
 Homo sapiens
angiotensin II
-
 Rattus norvegicus
angiotensin III
-
 Homo sapiens
angiotensin III
-
 Rattus norvegicus
bestatin 10% inhibition at 0.1 mM Homo sapiens
bestatin 35% inhibition at 0.1 mM Rattus norvegicus
Co2+ activation up to 50% at 0.001-0.0001 mM, inbhibitory above 0.002 mM Rattus norvegicus
E-64 i.e. L-trans-epoxysuccinyl-leucylamido(4-guanidino)butane, 10% inhibition at 0.1 mM Rattus norvegicus
EDTA 67% inhibition at 5 mM Homo sapiens
Gly-Gly-Phe-Leu fragment of Leu-enkephalin Homo sapiens
Gly-Gly-Phe-Leu fragment of Leu-enkephalin Rattus norvegicus
His-Phe-Arg-Trp fragment of alpha-melanocyte-stimulating hormone Homo sapiens
His-Phe-Arg-Trp fragment of alpha-melanocyte-stimulating hormone Rattus norvegicus
Leu-Trp-Met-Arg-Phe
-
 Homo sapiens
Leu-Trp-Met-Arg-Phe
-
 Rattus norvegicus
leupeptin 10% inhibition at 0.1 mM Homo sapiens
Lys-Val-Ile-Leu-Phe fragment of hydra head activator Homo sapiens
Lys-Val-Ile-Leu-Phe fragment of hydra ‘head activator’ Rattus norvegicus
Nalpha-tosyl-L-Phe-chloromethyl ketone 60% inhibition at 0.1 mM Rattus norvegicus
o-phenanthroline 93% inhibition at 1 mM Homo sapiens
o-phenanthroline 50% inhibition at 0.1 mM Rattus norvegicus
PMSF 40% inhibition at 1 mM Rattus norvegicus
probestin 85% inhibition at 0.1 mM Rattus norvegicus
Proctolin
-
 Homo sapiens
Proctolin
-
 Rattus norvegicus
puromycin 15% inhibition at 0.1 mM Rattus norvegicus
Tyr-Gly-Gly-Phe-Leu Leu-enkephalin Homo sapiens
Tyr-Gly-Gly-Phe-Leu Leu-enkephalin Rattus norvegicus
Tyr-Gly-Gly-Phe-Met Met-enkephalin Homo sapiens
Tyr-Gly-Gly-Phe-Met Met-enkephalin Rattus norvegicus
Zn2+ 50% inhibition at 0.032 mM Homo sapiens
Zn2+ 50% inhibition at 0.001 mM Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00621
-
 Arg-Arg-2-naphthylamide pH 7.4, 37°C Homo sapiens
0.00651
-
 Arg-Arg-2-naphthylamide pH 7.4, 37°C Rattus norvegicus
0.00788
-
 Arg-Arg-2-naphthylamide pH 8.0, 37°C Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activation up to 2fold at micromolar concentrations Homo sapiens
Co2+ activation up to 50% at 0.001-0.0001 mM, inbhibitory above 0.002 mM Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82500
-
 x * 82500, mass spectrometry Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
erythrocyte
-
 Homo sapiens
-
erythrocyte
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ala-Ala-2-naphthylamide + H2O poor substrate Homo sapiens Ala-Ala + 2-naphthylamine
-
 ?
Ala-Ala-2-naphthylamide + H2O poor substrate Rattus norvegicus Ala-Ala + 2-naphthylamine
-
 ?
Arg-Arg-2-naphthylamide + H2O
-
 Homo sapiens Arg-Arg + 2-naphthylamine
-
 ?
Arg-Arg-2-naphthylamide + H2O
-
 Rattus norvegicus Arg-Arg + 2-naphthylamine
-
 ?
Lys-Ala-4-methoxy-2-naphthylamide + H2O poor substrate Homo sapiens Lys-Ala + 4-methoxy-2-naphthylamine
-
 ?
Lys-Ala-4-methoxy-2-naphthylamide + H2O poor substrate Rattus norvegicus Lys-Ala + 4-methoxy-2-naphthylamine
-
 ?
additional information enzyme prefers a hydrophobic residue at P1’ position Homo sapiens ?
-
 ?
additional information enzyme prefers a hydrophobic residue at P1’ position Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
? x * 82500, mass spectrometry Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 45% of maximal activity Homo sapiens
7.4
-
 45% of maximal activity Rattus norvegicus
8
-
-
 Rattus norvegicus
8
-
 80% of maximal activity Homo sapiens
8.7 8.8
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000024
-
 angiotensin III 37°C, pH 7.4 Rattus norvegicus
0.00009
-
 angiotensin III 37°C, pH 7.4 Homo sapiens
0.00009
-
 Leu-Trp-Met-Arg-Phe 37°C, pH 7.4 Homo sapiens
0.00013
-
 Leu-Trp-Met-Arg-Phe 37°C, pH 7.4 Rattus norvegicus
0.00015
-
 Proctolin 37°C, pH 7.4 Homo sapiens
0.00021
-
 Lys-Val-Ile-Leu-Phe 37°C, pH 7.4 Homo sapiens
0.0004
-
 Lys-Val-Ile-Leu-Phe 37°C, pH 7.4 Rattus norvegicus
0.00043
-
 Proctolin 37°C, pH 7.4 Rattus norvegicus
0.00066
-
 angiotensin II 37°C, pH 7.4 Rattus norvegicus
0.00083
-
 angiotensin II 37°C, pH 7.4 Homo sapiens
0.0065
-
 His-Phe-Arg-Trp 37°C, pH 7.4 Homo sapiens
0.01
-
 His-Phe-Arg-Trp 37°C, pH 7.4 Rattus norvegicus
0.027
-
 Tyr-Gly-Gly-Phe-Leu 37°C, pH 7.4 Homo sapiens
0.027
-
 Tyr-Gly-Gly-Phe-Met 37°C, pH 7.4 Homo sapiens
0.0337
-
 Tyr-Gly-Gly-Phe-Leu 37°C, pH 7.4 Rattus norvegicus
0.0343
-
 Tyr-Gly-Gly-Phe-Met 37°C, pH 7.4 Rattus norvegicus
0.05
-
 Gly-Gly-Phe-Leu 37°C, pH 7.4 Homo sapiens
0.074
-
 Gly-Gly-Phe-Leu 37°C, pH 7.4 Rattus norvegicus