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Literature summary for 3.4.14.12 extracted from

  • Schulze, A.; Wermann, M.; Demuth, H.U.; Yoshimoto, T.; Ramsbeck, D.; Schlenzig, D.; Schilling, S.
    Continuous assays for meprin alpha and beta using prolyl tripeptidyl aminopeptidase (PtP) from Porphyromonas gingivalis (2018), Anal. Biochem., 559, 11-16 .
    View publication on PubMed
Search for more literature for 3.4.14.12

Application

Application Comment Organism
analysis continuous assay for endoprotease activity of meprin alpha and beta using prolyl tripeptidyl aminopeptidase and the chromogenic substrate KKGYVADAP-p-nitroanilide Porphyromonas gingivalis
synthesis strategy for expression and onestep-purification of the prolyl tripeptidyl peptidase Porphyromonas gingivalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Porphyromonas gingivalis

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Porphyromonas gingivalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Porphyromonas gingivalis the enzyme releases a tripeptide, X-X-P, from substrates exposing of a free N-terminus and a proline residue in the third position ?
-
 ?

Organism

Organism UniProt Comment Textmining
Porphyromonas gingivalis
-
-
-
Porphyromonas gingivalis Q7MUW6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-Asp-L-Ala-L-Pro-4-nitroanilide + H2O
-
 Porphyromonas gingivalis L-Asp-L-Ala-L-Pro + 4-nitroaniline
-
 ?
additional information the enzyme releases a tripeptide, X-X-P, from substrates exposing of a free N-terminus and a proline residue in the third position Porphyromonas gingivalis ?
-
 ?
additional information the cleavage of a meprin beta (EC 3.4.24.63) substrate leads to generation of the PtP substrate, and the activity of PtP results in release of a chromophore or fluorophore, coupled assay method evaluation, overview Porphyromonas gingivalis ?
-
 ?

Synonyms

Synonyms Comment Organism
prolyl tripeptidyl aminopeptidase
-
 Porphyromonas gingivalis
PTP
-
 Porphyromonas gingivalis
PtpA
-
 Porphyromonas gingivalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Porphyromonas gingivalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Porphyromonas gingivalis