Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain JM83 | Porphyromonas gingivalis |
into the pQE30 vector, lacking the region corresponding to residues 1-38, for transformation of Escherichia coli M15 cells | Porphyromonas gingivalis |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method, with 1.1 M potassium/sodium tartrate, 100 mM Ches buffer (pH 9.0), and 200 mM lithium sulfate | Porphyromonas gingivalis |
the structure of the ligand-free enzyme is solved at 2.1 A resolution, the structure of the S603A mutant-substrate complex is solved at 2.9 A resolution | Porphyromonas gingivalis |
Protein Variants | Comment | Organism |
---|---|---|
S603A | inactive | Porphyromonas gingivalis |
S603A | inactive mutant | Porphyromonas gingivalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
Gly-Ala-Pro-beta-naphthylamide | - |
Porphyromonas gingivalis | |
0.42 | - |
Gly-Ala-Pro-2-naphthylamide | in 20 mM Tris-HCl buffer (pH 7.0), at 37°C | Porphyromonas gingivalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
72500 | - |
2 * 72500, gel filtration | Porphyromonas gingivalis |
77000 | - |
determined by SDS-PAGE | Porphyromonas gingivalis |
145000 | - |
gel filtration | Porphyromonas gingivalis |
145000 | - |
determined by gel filtration | Porphyromonas gingivalis |
154000 | - |
SDS-PAGE | Porphyromonas gingivalis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Porphyromonas gingivalis | Q7MUW6 | - |
- |
Purification (Comment) | Organism |
---|---|
Toyopearl HW65C column chromatography and DEAE-Toyopearl column chromatography | Porphyromonas gingivalis |
using a Toyopearl HW65C and a DEAE-Toyopearl column | Porphyromonas gingivalis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.12 | - |
cell free extract | Porphyromonas gingivalis |
32.1 | - |
purification step, after protamine sulfate treatment | Porphyromonas gingivalis |
74.7 | - |
purification step, ammonium sulfate precipitation, 35-70% pellet | Porphyromonas gingivalis |
118 | - |
purification step, chromatography on a Toyopearl HW65C column | Porphyromonas gingivalis |
160 | - |
purification step, chromatography on a DEAE-Toyopearl column | Porphyromonas gingivalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ala-Phe-Pro-2-naphthylamide + H2O | - |
Porphyromonas gingivalis | Ala-Phe-Pro + 2-naphthylamine | - |
? | |
Ala-Phe-Pro-beta-naphthylamide + H2O | - |
Porphyromonas gingivalis | Ala-Phe-Pro + beta-naphthylamine | - |
? | |
Gly-Ala-Pro-2-naphthylamide + H2O | - |
Porphyromonas gingivalis | Gly-Ala-Pro + 2-naphthylamine | - |
? | |
Gly-Ala-Pro-beta-naphthylamide + H2O | - |
Porphyromonas gingivalis | Gly-Ala-Pro + beta-naphthylamine | - |
? | |
additional information | no activity with Pro-2-naphthylamide, Gly-Pro-2-naphthylamide, Z-Gly-Pro-2-naphthylamide, Z-Gly-Ala-Pro-2-naphthylamide, Z-Ala-Gly-Pro-2-naphthylamide, Gly-Ala-Gly-Pro-2-naphthylamide, Z-Gly-Ala-Gly-Pro-2-naphthylamide, and Ala-Gly-Ala-Gly-Pro-2-naphthylamide | Porphyromonas gingivalis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | - |
Porphyromonas gingivalis |
homodimer | 2 * 77000, SDS-PAGE | Porphyromonas gingivalis |
homodimer | 2 * 72500, gel filtration | Porphyromonas gingivalis |
Synonyms | Comment | Organism |
---|---|---|
prolyl tripeptidyl aminopeptidase | - |
Porphyromonas gingivalis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
- |
Porphyromonas gingivalis |
42 | - |
activity assay, Ala-Phe-Pro-beta-naphthylamide is used as a substrate | Porphyromonas gingivalis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
354 | - |
Gly-Ala-Pro-beta-naphthylamide | - |
Porphyromonas gingivalis | |
354 | - |
Gly-Ala-Pro-2-naphthylamide | in 20 mM Tris-HCl buffer (pH 7.0), at 37°C | Porphyromonas gingivalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Porphyromonas gingivalis |
7.2 | - |
activity assay, Ala-Phe-Pro-beta-naphthylamide is used as a substrate | Porphyromonas gingivalis |