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Literature summary for 3.4.14.11 extracted from

  • Miyabe, R.; Takahashi, Y.; Matsufuji, H.; Ogihara, J.; Ito, K.; Kawai, Y.; Masuda, T.; Suzuki, K.; Oda, M.
    Purification and partial characterization of an X-prolyl-dipeptidyl aminopeptidase from lactobacillus gasseri me-284 (2015), Food Sci. Technol. Res., 21, 445-451 .
No PubMed abstract available
Search for more literature for 3.4.14.11

Activating Compound

Activating Compound Comment Organism Structure
EDTA slight activation at 1.0 mM Lactobacillus gasseri
additional information no significant effect by 2-mercaptoethanol at 1 mM Lactobacillus gasseri

Inhibitors

Inhibitors Comment Organism Structure
Co2+ slight inhibition at 0.1-1.0 mM Lactobacillus gasseri
Cu2+ 85% inhibition at 1 mM Lactobacillus gasseri
diisopropylfluorophosphate 87% inhibition at 1 mM Lactobacillus gasseri
Diprotin A
-
 Lactobacillus gasseri
Hg2+ 98% inhibition at 1 mM Lactobacillus gasseri
Mg2+ slight inhibition at 0.1-1.0 mM Lactobacillus gasseri
additional information no significant effect by 2-mercaptoethanol at 1 mM Lactobacillus gasseri
Phenylmethylsulfonylfluoride 88.5% inhibition at 1 mM Lactobacillus gasseri
Zn2+ 56% inhibition at 1 mM Lactobacillus gasseri

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no significant effect by Ca2+ at 1 mM Lactobacillus gasseri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
173000
-
 gel filtration Lactobacillus gasseri

Organism

Organism UniProt Comment Textmining
Lactobacillus gasseri
-
-
-
Lactobacillus gasseri ATCC 33323
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 265fold in a seven step process, i.e, two steps of anion exchange chromatography, adsorption and hydrophobic interaction chromatography, two steps of gel filtration, and heparin affinity chromatography Lactobacillus gasseri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme is likely a serine proteinase Lactobacillus gasseri ?
-
 ?
additional information the enzyme is likely a serine proteinase Lactobacillus gasseri me-284 ?
-
 ?
additional information the enzyme is likely a serine proteinase Lactobacillus gasseri ATCC 33323 ?
-
 ?
Pro-Phe-Pro-Gly-Pro-Ile + H2O
-
 Lactobacillus gasseri Pro-Phe + Pro-Gly-Pro-Ile
-
 ?
Tyr-Pro-Phe-Pro-Gly-Pro-Ile + H2O i.e. BCM-7, beta-casomorphin 7 Lactobacillus gasseri Tyr-Pro + Phe-Pro-Gly-Pro-Ile
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 82000, SDS-PAGE Lactobacillus gasseri

Synonyms

Synonyms Comment Organism
LGAS_0712
-
 Lactobacillus gasseri
More see also EC 3.4.13.9 Lactobacillus gasseri
X-prolyl-dipeptidyl aminopeptidase
-
 Lactobacillus gasseri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
 assay at Lactobacillus gasseri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Lactobacillus gasseri

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0244
-
 pH 7.0, 55°C, recombinant enzyme Lactobacillus gasseri Diprotin A