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Literature summary for 3.4.14.11 extracted from

  • Stressler, T.; Eisele, T.; Schlayer, M.; Lutz-Wahl, S.; Fischer, L.
    Characterization of the recombinant exopeptidases PepX and PepN from Lactobacillus helveticus ATCC 12046 important for food protein hydrolysis (2013), PLoS ONE, 8, e70055.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.14.11

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 0.1 mM, 108% of initial activity Lactobacillus helveticus
KCl up to 300 mM, slight activitation. Inhibitory above 500 mM Lactobacillus helveticus
Na+ up to 300 mM, slight activitation. Inhibitory above 500 mM Lactobacillus helveticus

Application

Application Comment Organism
food industry X-prolyl dipeptidyl aminopeptidase PepX, EC 3.4.14.11, and the general aminopeptidase N, EC 3.4.11.2, exhibit a clear synergistic effect in casein hydrolysis studies. Here, the relative degree of hydrolysis is increased by approx. 132% Lactobacillus helveticus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Esherichia coli Lactobacillus helveticus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ 1 mM, 89% residual activity Lactobacillus helveticus
Cu2+ 1 mM, 21% residual activiy Lactobacillus helveticus
Hg2+ 0.001 mM, 3% residual activity Lactobacillus helveticus
KCl up to 300 mM, slight activitation. Inhibitory above 500 mM Lactobacillus helveticus
L-Phe-L-Pro 10 mM, 38% inhibition, mixed-ype inhibition Lactobacillus helveticus
L-Tyr-L-Pro 10 mM, 28% inhibition, mixed-type inhibition Lactobacillus helveticus
Na+ up to 300 mM, slight activitation. Inhibitory above 500 mM Lactobacillus helveticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 Gly-L-Pro 4-nitroanilide pH 6.5, 37°C Lactobacillus helveticus
1.3
-
 L-Ala-L-Pro 4-nitroanilide pH 6.5, 37°C Lactobacillus helveticus
1.5
-
 L-Arg-L-Pro 4-nitroanilide pH 6.5, 37°C Lactobacillus helveticus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 2 * 90000, SDS-PAGE Lactobacillus helveticus
180000
-
 PAGE Lactobacillus helveticus

Organic Solvent Stability

Organic Solvent Comment Organism
Acetone 10% v/v, 50% residual activity Lactobacillus helveticus
Ethanol 10% v/v, 78% residual activity Lactobacillus helveticus

Organism

Organism UniProt Comment Textmining
Lactobacillus helveticus S4TJ23
-
-
Lactobacillus helveticus ATCC 120460 S4TJ23
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Gly-L-Pro 4-nitroanilide + H2O 6% of the activity with L-Ala-L-Pro-4-nitroanilide Lactobacillus helveticus Gly-L-Pro + 4-nitroaniline
-
 ?
Gly-L-Pro 4-nitroanilide + H2O 6% of the activity with L-Ala-L-Pro-4-nitroanilide Lactobacillus helveticus ATCC 120460 Gly-L-Pro + 4-nitroaniline
-
 ?
L-Ala-L-Pro 4-nitroanilide + H2O
-
 Lactobacillus helveticus L-Ala-L-Pro + 4-nitroaniline
-
 ?
L-Ala-L-Pro 4-nitroanilide + H2O
-
 Lactobacillus helveticus ATCC 120460 L-Ala-L-Pro + 4-nitroaniline
-
 ?
L-Arg-L-Pro 4-nitroanilide + H2O 59% of the activity with L-Ala-L-Pro-4-nitroanilide Lactobacillus helveticus L-Arg-L-Pro + 4-nitroaniline
-
 ?
L-Arg-L-Pro 4-nitroanilide + H2O 59% of the activity with L-Ala-L-Pro-4-nitroanilide Lactobacillus helveticus ATCC 120460 L-Arg-L-Pro + 4-nitroaniline
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 90000, SDS-PAGE Lactobacillus helveticus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Lactobacillus helveticus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
65
-
 8% residual activity Lactobacillus helveticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 15 days, 90% residual activity Lactobacillus helveticus
50
-
 15 days, 34% residual activity Lactobacillus helveticus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 Na2HPO4/KH2PO4 buffer Lactobacillus helveticus

pH Range

pH Minimum pH Maximum Comment Organism
7
-
 87% of maximum activity, Na2HPO4/KH2PO4 buffer Lactobacillus helveticus